Carvalho-Sombra T C F, Fernandes D D, Bezerra B M O, Nunes-Pinheiro D C S
Veterinary Science Post Graduate Program (PPGCV), College of Veterinary Sciences (FAVET), State University of Ceará (UECE), Brazil.
Department of Veterinary Medicine, University of Fortaleza (UNIFOR), Ceará, Brazil.
Vet Anim Sci. 2021 Jan 8;11:100165. doi: 10.1016/j.vas.2021.100165. eCollection 2021 Mar.
Systemic parameters and its relationship with Somatic Cell Count (SCC) in dairy cows were evaluated. Cows that presented subclinical mastitis (SMC; = 16) and healthy cows (HC; = 6) were selected and identified by the and SCC in milk. SCC results were logarithmically transformed into somatic cell score (SCS). HC presented SCS 〈 4.32 while SMC presented SCS 〉 4.32. Milk and blood samples were collected in three days: D1 (first day of sampling), D2 (48 h after D1) and D3 (7 days after D1), to determine White Blood Cells (WBC), albumin, total protein, total bilirubin and malondialdehyde (MDA). Results were expressed as mean ± standard deviation. It was considered significant at < 0.05. The data of SCS on D1, D2 and D3 in SMC were: 6.8 ± 1.7, 6.4 ± 1.8, and 6.3 ± 2.0, respectively. In SMC the MDA (nmolL) were: D1 - 9.3 ± 2.6, D2 - 8.6 ± 2.4, and D3 - 11.5 ± 3.5. The MDA on D3 in SMC (11.5 ± 3.5) were increase when compared to HC (6.0 ± 1.3) ( < 0.001). No significant difference was found in WBC, TP, ALB, and TB between groups. It was observed a positive correlation between MDA-SCS (ρ = 0.4) and between WBC-SCS (ρ = 0.3) in the SMC group. It was concluded that the systemic repercussion damage in the mammary gland promoted by subclinical mastitis in dairy cows can be assessed using the MDA and WBC biomarkers.
评估了奶牛的全身参数及其与体细胞计数（SCC）的关系。通过牛奶中的SCC选择并鉴定出患有亚临床乳腺炎（SMC；n = 16）的奶牛和健康奶牛（HC；n = 6）。SCC结果经对数转换为体细胞评分（SCS）。HC的SCS〈4.32，而SMC的SCS〉4.32。在三天内采集牛奶和血液样本：D1（采样第一天）、D2（D1后48小时）和D3（D1后7天），以测定白细胞（WBC）、白蛋白、总蛋白、总胆红素和丙二醛（MDA）。结果以平均值±标准差表示。P<0.05被认为具有显著性。SMC组D1、D2和D3的SCS数据分别为：6.8±1.7、6.4±1.8和6.3±2.0。在SMC中，MDA（nmol/L）分别为：D1 - 9.3±2.6、D2 - 8.6±2.4和D3 - 11.5±3.5。与HC（6.0±1.3）相比，SMC组D3的MDA（11.5±3.5）升高（P<0.001）。两组之间在WBC、TP、ALB和TB方面未发现显著差异。在SMC组中观察到MDA - SCS之间呈正相关（ρ = 0.4）以及WBC - SCS之间呈正相关（ρ = 0.3）。得出的结论是，奶牛亚临床乳腺炎引发的乳腺全身反应性损伤可以使用MDA和WBC生物标志物进行评估。
