Keenan Christine R, Mlodzianoski Michael J, Coughlan Hannah D, Bediaga Naiara G, Naselli Gaetano, Lucas Erin C, Wang Qike, de Graaf Carolyn A, Hilton Douglas J, Harrison Leonard C, Smyth Gordon K, Rogers Kelly L, Boudier Thomas, Allan Rhys S, Johanson Timothy M
The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.
Department of Medical Biology, The University of Melbourne, Parkville, VIC, 3010, Australia.
iScience. 2021 Feb 7;24(3):102161. doi: 10.1016/j.isci.2021.102161. eCollection 2021 Mar 19.
The proximity pattern and radial distribution of chromosome territories within spherical nuclei are random and non-random, respectively. Whether this distribution pattern is conserved in the partitioned or lobed nuclei of polymorphonuclear cells is unclear. Here we use chromosome paint technology to examine the chromosome territories of all 46 chromosomes in hundreds of single human neutrophils - an abundant and famously polymorphonuclear immune cell. By comparing the distribution of chromosomes to randomly shuffled controls and validating with orthogonal chromosome conformation capture technology, we show for the first time that human chromosomes randomly distribute to neutrophil nuclear lobes, while maintaining a non-random radial distribution within these lobes. Furthermore, we demonstrate that chromosome length correlates with three-dimensional volume not only in neutrophils but other human immune cells. This work demonstrates that chromosomes are largely passive passengers during the neutrophil lobing process but are able to subsequently maintain their macro-level organization within lobes.
在球形细胞核内，染色体区域的邻近模式和径向分布分别是随机的和非随机的。尚不清楚这种分布模式在多形核细胞的分隔核或叶状核中是否保守。在这里，我们使用染色体涂染技术检查了数百个单个人类中性粒细胞（一种丰富且著名的多形核免疫细胞）中所有46条染色体的染色体区域。通过将染色体的分布与随机重排的对照进行比较，并用正交染色体构象捕获技术进行验证，我们首次表明人类染色体随机分布到中性粒细胞核叶中，同时在这些核叶内保持非随机的径向分布。此外，我们证明染色体长度不仅在中性粒细胞中，而且在其他人类免疫细胞中都与三维体积相关。这项工作表明，染色体在中性粒细胞分叶过程中很大程度上是被动的乘客，但随后能够在核叶内维持其宏观水平的组织。
