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没食子酸乙酯含量高的（L.）叶提取物对人癌细胞系 KB 的抗增殖作用。

Antiproliferative effect of (L.) leaf extract rich in ethyl gallate against human carcinoma cell line KB.

机构信息

Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.

Division of Plant Physiology and Biochemistry, Indian Institute of Horticultural Research, Bengaluru, Karnataka, India.

出版信息

Indian J Pharmacol. 2020 Nov-Dec;52(6):488-494. doi: 10.4103/ijp.IJP_223_17.

DOI:10.4103/ijp.IJP_223_17
PMID:33666190
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8092180/
Abstract

OBJECTIVES

The objective of this study is to analyze the antiproliferative activity of Acacia nilotica (L.) leaf ethanolic extract against cancer KB cells and to determine the mode of cancer cytotoxicity.

MATERIALS AND METHODS

In this study, high-performance liquid chromatography and liquid chromatography-mass spectrometry analysis were done to confirm the presence of ethyl gallate as a major bioactive phenolic in the leaf ethanolic extract of A. nilotica, further dose-dependent (0-120 μg/mL) antiproliferative effect was investigated in human carcinoma cell line KB. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, reactive oxygen species, mitochondrial membrane potential loss, DNA damage, and apoptosis were evaluated.

RESULTS

A. nilotica leaf ethanolic extract (ANLEE) showed effective concentration (EC) of 40 μg/mL. Interference of growth was significantly (P < 0.05) high in KB cells treated with ANLEE when compared to untreated control, but less when compared to the reference drug paclitaxel. In addition, the in vivo acute toxicity study demonstrated the safe limit of administration of 2000 mg/kg body weight ANLEE by the histological analysis in rats. The results from the present study indicate that mitochondria and DNA of KB cells are severely affected leading to apoptosis.

CONCLUSIONS

ANLEE is a prospective source for cancer therapy and therefore should be highlighted to explore on its wide range of safety in rats and efficacy against human carcinoma cell line KB.

摘要

目的

本研究旨在分析金合欢（L.）叶乙醇提取物对癌症 KB 细胞的抗增殖活性，并确定其抗癌细胞毒性的模式。

材料和方法

在这项研究中，进行了高效液相色谱法和液相色谱-质谱分析，以确认乙基没食子酸作为金合欢叶乙醇提取物中的主要生物活性酚类物质的存在，进一步研究了其在人癌细胞系 KB 中的剂量依赖性（0-120μg/mL）抗增殖作用。使用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐、活性氧物种、线粒体膜电位丧失、DNA 损伤和细胞凋亡进行评估。

结果

金合欢叶乙醇提取物（ANLEE）显示出 40μg/mL 的有效浓度（EC）。与未处理的对照相比，用 ANLEE 处理的 KB 细胞的生长受到显著（P<0.05）的干扰，但与参考药物紫杉醇相比，这种干扰较小。此外，体内急性毒性研究表明，通过大鼠的组织学分析，2000mg/kg 体重 ANLEE 的给药安全限量。本研究的结果表明，KB 细胞的线粒体和 DNA 受到严重影响，导致细胞凋亡。

结论

ANLEE 是癌症治疗的有前途的来源，因此应该强调其在大鼠中的广泛安全性和对人癌细胞系 KB 的疗效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/7bb5c6b4fc4f/IJPharm-52-488-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/75b32c05f8a3/IJPharm-52-488-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/892bfb15b69d/IJPharm-52-488-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/09db1eeec0bb/IJPharm-52-488-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/563de87cba91/IJPharm-52-488-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/e4cd834f12df/IJPharm-52-488-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/7bb5c6b4fc4f/IJPharm-52-488-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/75b32c05f8a3/IJPharm-52-488-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/892bfb15b69d/IJPharm-52-488-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/ad093e7d31e5/IJPharm-52-488-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/1a87447fd204/IJPharm-52-488-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/8a6ca035a22c/IJPharm-52-488-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/09db1eeec0bb/IJPharm-52-488-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/563de87cba91/IJPharm-52-488-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/e4cd834f12df/IJPharm-52-488-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0244/8092180/7bb5c6b4fc4f/IJPharm-52-488-g009.jpg

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