School of Bio Sciences and Technology, VIT University, Vellore 632014, Tamil Nadu, India.
Evid Based Complement Alternat Med. 2011;2011:274741. doi: 10.1093/ecam/neq060. Epub 2011 Jun 15.
Dietary intake of phytochemicals having antioxidant activity is associated with a lower risk of mortality from many diseases. Therefore, the aim of this study was to determine the free radical scavenging, cytotoxic and hemolytic activities of leaves of Acacia nilotica by using various methods. The results of the present study revealed that ethanol extract was the most effective and IC(50) value was found to be 53.6 μg mL(-1) for Vero cell lines and 28.9 μg mL(-1) for Hela cell lines in cytotoxicity assays. The zone of color retention was 14.2 mm in β-carotene bleaching assay, which was as significant as positive control, butylated hydroxy toluene. None of the tested extracts possessed any hemolytic activity against rat and human erythrocytes revealing their cytotoxic mechanism and non-toxicity. Thus, only the ethanol extract could be considered as a potential source of anticancer and antioxidant compounds. Further phytochemical studies will be performed for specification of the biologically active principles.
具有抗氧化活性的植物化学物质的饮食摄入与许多疾病的死亡率降低有关。因此，本研究的目的是使用各种方法来确定金合欢树叶的自由基清除、细胞毒性和溶血活性。本研究的结果表明，在细胞毒性测定中，乙醇提取物对 Vero 细胞系的 IC 50 值为 53.6μg/mL，对 Hela 细胞系的 IC 50 值为 28.9μg/mL，是最有效的；β-胡萝卜素褪色试验中颜色保留区为 14.2mm，与阳性对照丁羟甲苯相当。在溶血试验中，没有一种提取物对大鼠和人红细胞具有任何溶血活性，这表明它们的细胞毒性机制是无毒的。因此，只有乙醇提取物可以被认为是抗癌和抗氧化化合物的潜在来源。将进行进一步的植物化学研究以确定具有生物活性的原则。
