相思树皮提取物对口腔细菌、葡糖基转移酶的体外活性及抗氧化作用
In vitro activities of Acacia nilotica (L.) Delile bark fractions against Oral Bacteria, Glucosyltransferase and as antioxidant.
机构信息
School of Molecular Life Science, Faculty of Applied Biological Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Department of Horticulture, Faculty of Agriculture, University of Khartoum, Khartoum North-Shambat, Sudan.
出版信息
BMC Complement Med Ther. 2020 Nov 23;20(1):360. doi: 10.1186/s12906-020-03147-4.
BACKGROUND
Dental caries and periodontal disease are the most common chronic infectious oral diseases in the world. Acacia nilotica was commonly known in Sudan as Garad or Sunt has a wide range of medicinal uses. In the present study, antibacterial activity of oral bacteria (Streptococcus sobrinus and Porphyromonas gingivalis), inhibitory activity against glucosyltransferase (GTF) enzyme and antioxidant activity were assayed for methanolic crude extract of A. nilotica bark and its fractions.
METHODS
Methanoilc crude extract of A. nilotica bark was applied to a Sephadex LH-20 column and eluted with methanol, aqueous methanol, and finally aqueous acetone to obtain four fractions (Fr1- Fr4). Furthermore, the crude extract and fractions were subjected to analytical high performance liquid chromatography (HPLC). The crude extract and its fractions were assayed for antibacterial activity against S. sobrinus and P. gingivalis using a microplate dilution assay method to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), as well as GTF inhibition and antioxidant activity using ABTS radical scavenging method.
RESULTS
Fractions (Fr1 and Fr2) exhibited MIC values of 0.3 mg/ml against the P. gingivalis. Additionally, Fr2 displayed MBC value of 1 mg/ml against two types of bacteria. Fr4 showed an especially potent GTF inhibitory activity with IC value of 3.9 μg/ml. Fr1 displayed the best antioxidant activity with IC value of 1.8 μg/ml. The main compound in Fr1 was identified as gallic acid, and Fr2 was mostly a mixture of gallic acid and methyl gallate.
CONCLUSIONS
The results obtained in this study provide some scientific rationale and justify the use of this plant for the treatment of dental diseases in traditional medicine. A. nilotica bark, besides their antibacterial potentiality and GTF inhibitory activity, it may be used as adjuvant antioxidants in mouthwashes. Further studies in the future are required to identify the rest of the active compounds.
背景
龋齿和牙周病是世界上最常见的慢性传染性口腔疾病。在苏丹，金合欢通常被称为 Garad 或 Sunt，具有广泛的药用用途。在本研究中，测定了金合欢树皮及其馏分的甲醇粗提取物对口腔细菌（变形链球菌和牙龈卟啉单胞菌）的抗菌活性、对葡糖基转移酶（GTF）酶的抑制活性和抗氧化活性。
方法
将金合欢树皮的甲醇粗提取物应用于 Sephadex LH-20 柱，并用甲醇、甲醇水溶液和最后用丙酮水溶液洗脱，得到四个馏分（Fr1-Fr4）。此外，对粗提取物和馏分进行分析高效液相色谱（HPLC）分析。采用微量稀释法测定金合欢树皮甲醇粗提取物及其馏分对变形链球菌和牙龈卟啉单胞菌的抗菌活性，以确定最小抑菌浓度（MIC）和最小杀菌浓度（MBC），并采用 ABTS 自由基清除法测定 GTF 抑制活性和抗氧化活性。
结果
馏分（Fr1 和 Fr2）对牙龈卟啉单胞菌的 MIC 值为 0.3mg/ml。此外，Fr2 对两种细菌的 MBC 值为 1mg/ml。Fr4 对 GTF 具有特别强的抑制活性，IC 值为 3.9μg/ml。Fr1 表现出最好的抗氧化活性，IC 值为 1.8μg/ml。Fr1 中的主要化合物被鉴定为没食子酸，Fr2 主要是没食子酸和甲基没食子酸的混合物。
结论
本研究结果为该植物在传统医学中治疗口腔疾病提供了一些科学依据。金合欢树皮除了具有抗菌潜力和 GTF 抑制活性外，还可作为漱口水的辅助抗氧化剂。未来需要进一步研究以确定其余的活性化合物。
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