Beckman Laser Institute & Medical Clinic, University of California, Irvine, California, U.S.A.
Department of Dermatology, Korea University Anam Hospital, Seoul, South Korea.
Laryngoscope. 2021 Jul;131(7):E2196-E2203. doi: 10.1002/lary.29461. Epub 2021 Mar 5.
To examine the acid-base and histological changes in in vivo rabbit cutaneous tissue after electrochemical therapy.
In vivo rabbit tissue study.
The shaved skin on the backs of female Oryctolagus cuniculi were assigned to treatments with or without tumescence with normal saline. Two platinum-needle electrodes were inserted into each treatment area and connected to a direct current (DC) power supply. Voltage (3-5 V) was varied and applied for 5 minutes. The wound-healing process was monitored via digital photography and ultrasonography until euthanasia at day 29. Treatment areas were biopsied, and specimens were sectioned through a sagittal midline across both electrode insertion sites. Samples were then evaluated utilizing light microscopy (hematoxylin and eosin, Masson's Trichrome, and Picrosirius red).
Treatment sites developed mild inflammation that dissipated at lower voltages or became scabs at higher voltages. Ultrasonography demonstrated acoustic shadowing with spatial spread that increased with increasing voltage application. The 4- and 5-V sites treated with saline had localized areas of increased tissue density at day 29. Although specimens treated with 3 V did not look significantly different from control tissue, 4- and 5-V samples with and without saline tumescence had finer, less-organized collagen fibers and increased presence of fibrocytes and inflammatory infiltrates.
Electrochemical therapy caused localized injury to in vivo rabbit cutaneous tissue, prompting regenerative wound repair. With future development, this technology may offer precise, low-cost rejuvenation to restore the functionality and appearance of dermal scars and keloids.
NA Laryngoscope, 131:E2196-E2203, 2021.
研究电化学疗法后活体兔皮组织的酸碱变化和组织学变化。
体内兔组织研究。
将雌性豚鼠背部剃毛的皮肤分为盐水肿胀组和非肿胀组。将两根铂针电极插入每个治疗区域，并连接到直流电（DC）电源。改变电压（3-5V）并应用 5 分钟。通过数字摄影和超声监测伤口愈合过程，直至第 29 天安乐死。对治疗区域进行活检，并通过矢状中线穿过两个电极插入部位对标本进行切片。然后利用光镜（苏木精和伊红、马松三色和苦味酸天狼猩红）评估样本。
治疗部位出现轻度炎症，在较低电压下消散，在较高电压下形成结痂。超声显示随着电压应用的增加，空间扩散的声影增加。用生理盐水处理的 4V 和 5V 部位在第 29 天有局部组织密度增加的区域。尽管用 3V 处理的标本与对照组织没有明显差异，但用生理盐水肿胀和非肿胀的 4V 和 5V 样本具有更细、组织更无序的胶原纤维，以及更多纤维细胞和炎症浸润。
电化学疗法导致活体兔皮组织局部损伤，引发再生性伤口修复。随着未来的发展，这项技术可能会提供精确的、低成本的皮肤年轻化治疗，以恢复真皮疤痕和瘢痕疙瘩的功能和外观。
无 喉科学，131：E2196-E2203，2021。
