Hutchison Dana M, Hakimi Amir A, Wijayaweera Avin, Seo Soohong, Hong Ellen M, Pham Tiffany T, Bircan Melissa, Sivoraphonh Ryan, Dunn Brandyn, Kobayashi Mark R, Kim Sehwan, Wong Brian Jf
Beckman Laser Institute & Medical Clinic, University of California - Irvine, Irvine, CA, USA.
Department of Dermatology, Korea University Anam Hospital, Seoul, South Korea.
Scars Burn Heal. 2021 Mar 16;7:2059513120988532. doi: 10.1177/2059513120988532. eCollection 2021 Jan-Dec.
Scar treatments aim to address pathologic collagen deposition; however, they can be expensive or difficult to control. Electrochemical therapy (ECT) offers a simple alternative treatment. The purpose of this study is to examine the acid-base and histological changes in ex vivo human abdominal skin following ECT.
Forty-two ex vivo human panniculus tissue sections collected from six individuals were tumesced with normal saline. ECT was performed by inserting two platinum needle electrodes connected to a DC power supply into each specimen. Voltage was varied (3-6 V) and applied for 5 minutes. Each specimen was sectioned across both electrode insertion sites and immediately stained with pH sensitive dye. The width of dye color change for each dosimetry pair was calculated. Hematoxylin and eosin staining was used to evaluate samples.
ECT caused a spatially localised and dose-dependent increased area of acidic and basic pH around the anode and cathode, respectively. A significantly greater mean width of pH change was generated at the cathode compared to the anode in all treatment groups. Histological evaluation displayed broad condensation and hyalinisation of dermal collagen.
ECT triggered dermal pH alterations and changed the underlying structural framework of the specimen. This technology may serve as a low-cost, minimally invasive local soft-tissue remodeling technique with potential application in scar management.
Electrochemical therapy is a novel treatment that causes spatially selective dermal injury in areas of interest. This study measures the effects of electrochemical therapy when applied to abdominal skin. Electrochemical therapy appears to have beneficial effects by causing a highly localised reduction in collagen content or local softening of tissue, which is consistent with other studies on scar therapies, including chemexfoliation, radiofrequency technologies, and lasers. However, electrochemical therapy can be performed at a fraction of the costs of these aforementioned modalities.
瘢痕治疗旨在解决病理性胶原蛋白沉积问题；然而，这些治疗方法可能成本高昂或难以控制。电化学疗法（ECT）提供了一种简单的替代治疗方法。本研究的目的是检查ECT后离体人腹部皮肤的酸碱和组织学变化。
从6名个体收集的42个离体人皮下组织切片用生理盐水肿胀。通过将连接到直流电源的两根铂针电极插入每个标本中进行ECT。改变电压（3 - 6 V）并施加5分钟。每个标本在两个电极插入部位进行切片，并立即用pH敏感染料染色。计算每个剂量组对的染料颜色变化宽度。使用苏木精和伊红染色评估样本。
ECT分别在阳极和阴极周围引起酸性和碱性pH值在空间上局部化且剂量依赖性增加的区域。在所有治疗组中，阴极处产生的pH变化平均宽度明显大于阳极处。组织学评估显示真皮胶原广泛凝聚和玻璃样变。
ECT引发真皮pH改变并改变标本的基础结构框架。该技术可作为一种低成本、微创的局部软组织重塑技术，在瘢痕管理中具有潜在应用价值。
5。
电化学疗法是一种新型治疗方法，可在感兴趣的区域引起空间选择性真皮损伤。本研究测量了电化学疗法应用于腹部皮肤时的效果。电化学疗法似乎具有有益效果，通过引起胶原蛋白含量高度局部降低或组织局部软化，这与其他瘢痕治疗研究一致，包括化学剥脱、射频技术和激光。然而，电化学疗法的成本仅为上述方法的一小部分。
