Institute of Computer Science, Warsaw University of Technology, Warsaw, Poland.
University of Warmia and Mazury, Olsztyn, Poland.
Sci Data. 2019 Dec 3;6(1):302. doi: 10.1038/s41597-019-0311-3.
Despite the use of Hymenolepis diminuta as a model organism in experimental parasitology, a full genome description has not yet been published. Here we present a hybrid de novo genome assembly based on complementary sequencing technologies and methods. The combination of Illumina paired-end, Illumina mate-pair and Oxford Nanopore Technology reads greatly improved the assembly of the H. diminuta genome. Our results indicate that the hybrid sequencing approach is the method of choice for obtaining high-quality data. The final genome assembly is 177 Mbp with contig N50 size of 75 kbp and a scaffold N50 size of 2.3 Mbp. We obtained one of the most complete cestode genome assemblies and annotated 15,169 potential protein-coding genes. The obtained data may help explain cestode gene function and better clarify the evolution of its gene families, and thus the adaptive features evolved during millennia of co-evolution with their hosts.
尽管细粒棘球绦虫被用作实验寄生虫学的模式生物，但尚未发表其完整的基因组描述。在这里，我们基于互补测序技术和方法呈现了一个混合从头基因组组装。Illumina 配对末端、Illumina mate-pair 和 Oxford Nanopore Technology 读长的组合极大地改进了细粒棘球绦虫基因组的组装。我们的结果表明，混合测序方法是获得高质量数据的首选方法。最终的基因组组装大小为 177Mb，contig N50 大小为 75kbp，scaffold N50 大小为 2.3Mb。我们获得了最完整的绦虫基因组组装之一，并注释了 15169 个潜在的蛋白质编码基因。获得的数据可能有助于解释绦虫基因的功能，并更好地阐明其基因家族的进化，从而阐明其在与宿主数千万年的共同进化过程中所产生的适应性特征。
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