Department of Pathology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230036, Anhui, China.
Intelligent Pathology Institute, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230036, Anhui, China.
Brain Tumor Pathol. 2021 Apr;38(2):122-131. doi: 10.1007/s10014-021-00395-1. Epub 2021 Mar 5.
Meningeal solitary fibrous tumor (SFT) and hemangiopericytoma (HPC) were categorized as the same entity in the World Health Organization (WHO) 2016 classification of tumors of the central nervous system (CNS). Although NAB2-STAT6 fusion protein can be used to distinguish most of SFT/HPC from the other sarcomas, additional biomarkers were requested to separate meningeal SFT/HPC from meningioma and the molecular pathological difference between meningeal SFT/HPC and extra-CNS SFT/HPC still remains unclear. In this study, we evaluated the expression of TTF-1 in 67 meningeal SFT/HPC, 62 extra-CNS SFT/HPC and 201 meningiomas samples with immunohistochemistry (IHC) assays. The results showed that TTF-1 was detected in 23 of 67 (34.3%) meningeal SFT/HPC, 3 retroperitoneum SFT/HPC and none of meningiomas. Meanwhile, the copy number variation and mRNA expression of TTF-1 were measured by real-time quantitative PCR (qPCR) in meningeal SFT/HPC. These results demonstrated that TTF-1 protein expression level was significantly correlated with its transcription level, but independently related to the gene copy number variant. In conclusion, our study suggested that a large proportion of meningeal SFT/HPC was positive to TTF-1, while very few extra CNS SFT/HPC cases and no meningiomas were stained. So TTF-1 has value as an auxiliary diagnostic marker for meningeal SFT/HPC.
脑脊膜孤立性纤维瘤（SFT）和血管外皮细胞瘤（HPC）在 2016 年世界卫生组织（WHO）中枢神经系统（CNS）肿瘤分类中被归为同一实体。虽然 NAB2-STAT6 融合蛋白可用于区分大多数 SFT/HPC 与其他肉瘤，但仍需要额外的生物标志物来区分脑脊膜 SFT/HPC 与脑膜瘤，以及脑脊膜 SFT/HPC 与中枢神经系统外 SFT/HPC 之间的分子病理差异仍不清楚。在这项研究中，我们通过免疫组织化学（IHC）检测评估了 67 例脑脊膜 SFT/HPC、62 例中枢神经系统外 SFT/HPC 和 201 例脑膜瘤样本中 TTF-1 的表达。结果显示，TTF-1 在 67 例脑脊膜 SFT/HPC 中的 23 例、3 例腹膜后 SFT/HPC 中检测到，但在脑膜瘤中均未检测到。同时，通过实时定量 PCR（qPCR）测量了脑脊膜 SFT/HPC 中 TTF-1 的拷贝数变异和 mRNA 表达。这些结果表明，TTF-1 蛋白表达水平与其转录水平显著相关，但与基因拷贝数变异独立相关。总之，我们的研究表明，相当一部分脑脊膜 SFT/HPC 对 TTF-1 呈阳性，而很少有中枢神经系统外 SFT/HPC 病例和脑膜瘤呈阳性染色。因此，TTF-1 作为脑脊膜 SFT/HPC 的辅助诊断标志物具有一定价值。
