Guo Zhen, Wang Yujiao, Tan Tingyuan, Ji Yuwen, Hu Jun, Zhang Yi
Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Zhangjiang Lab, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
ACS Biomater Sci Eng. 2021 Apr 12;7(4):1703-1712. doi: 10.1021/acsbiomaterials.1c00187. Epub 2021 Mar 5.
Biofilms are widely involved in human lives, such as in medical infection, environmental remediation, and industrial processes. However, the control of the biofilm has still been a challenge because of its strong drug resistance. Here, we designed and synthesized an amphipathic antimicrobial peptide (Ac-KHHQKLVFFAK-NH (KKd-11)) that was composed of d-amino acids (DAAs). KKd-11 was found to self-assemble into a hydrogel with an improved long-term antimicrobial ability and a better antiprotease activity as compared to the hydrogel formed by Ac-KHHQKLVFFAK-NH (KK-11). Our results indicated that KKd-11 was not only able to inhibit the formation of biofilms but also could effectively damage preformed mature biofilms and kill the bacteria within the biofilms. Besides, cell viability assays indicated that the KKd-11 peptide had very good biocompatibility. We think d-peptide hydrogels may have great potential in the treatment of biofilm-induced infections.
生物膜广泛存在于人类生活中，例如在医学感染、环境修复和工业过程中。然而，由于生物膜具有很强的耐药性，对其进行控制仍然是一项挑战。在此，我们设计并合成了一种由d-氨基酸（DAA）组成的两亲性抗菌肽（Ac-KHHQKLVFFAK-NH (KKd-11)）。与由Ac-KHHQKLVFFAK-NH (KK-11)形成的水凝胶相比，发现KKd-11能自组装成具有改善的长期抗菌能力和更好的抗蛋白酶活性的水凝胶。我们的结果表明，KKd-11不仅能够抑制生物膜的形成，还能有效破坏预先形成的成熟生物膜并杀死生物膜内的细菌。此外，细胞活力测定表明KKd-11肽具有非常好的生物相容性。我们认为d-肽水凝胶在治疗生物膜引起的感染方面可能具有巨大潜力。
