School of Life Sciences, Zhengzhou University, Zhengzhou, China.
R&D Department, Henan Zhongze Biological Engineering Co. LTD, Zhengzhou, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2021 Apr;38(4):594-601. doi: 10.1080/19440049.2021.1876251. Epub 2021 Mar 5.
The direct and indirect competitive fluorescence-linked immunosorbent assay (FLISA and icFLISA) incorporating quantum dots (QDs) for the detection of fleroxacin (FLE) was established for the first time in this study. The monoclonal antibody specific for FLE was successfully conjugated with QDs after purification by the caprylic acid-ammonium sulphate method. The limits of detection of FLISA and icFLISA were 0.012 ng/mL and 0.006 ng/mL, respectively; IC were 0.32 ng/mL and 0.19 ng/mL; and the detection ranges were 0.012-24.490 ng/mL and 0.006-16.210 ng/mL. The recovery was 93.8%-112.4% and the coefficient of variation was below 11.75%. The fabricated FLISA and icFLISA are cost-effective, high sensitive and can be an alternative method in the detection of FLE residues.
本研究首次建立了基于量子点（QDs）的直接和间接竞争荧光酶联免疫吸附测定法（FLISA 和 icFLISA），用于检测氟罗沙星（FLE）。经辛酸-硫酸铵法纯化后，将针对 FLE 的单克隆抗体成功偶联到 QDs 上。FLISA 和 icFLISA 的检测限分别为 0.012ng/mL 和 0.006ng/mL；IC 分别为 0.32ng/mL 和 0.19ng/mL；检测范围分别为 0.012-24.490ng/mL 和 0.006-16.210ng/mL。回收率为 93.8%-112.4%，变异系数低于 11.75%。所制备的 FLISA 和 icFLISA 具有成本效益，高灵敏度，可作为检测 FLE 残留的替代方法。
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