School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
Luminescence. 2022 Aug;37(8):1300-1308. doi: 10.1002/bio.4296. Epub 2022 Jun 6.
Bacitracin zinc (BAC), a polypeptide antibiotic, is utilized as a feed additive due to its ability to promote growth in animals. However, the abuse of BAC can lead to a great threat to food safety. Therefore, there is an urgent need to develop a rapid and sensitive detection method. In this study, a monoclonal antibody (mAb) against BAC with excellent sensitivity and specificity was obtained. For the first time, quantum dots (QDs) were conjugated with the prepared mAb against BAC and rabbit anti-mouse antibody to fabricate a direct and an indirect competitive fluorescence-linked immunosorbent assay (dc-FLISA and ic-FLISA) to detect BAC. The IC of dc-FLISA and ic-FLISA were 0.28 ng/ml and 0.17 ng/ml, respectively. The limits of detection were 0.0016 ng/ml and 0.001 ng/ml, respectively, and the detection ranges were 0.0016-46.50 ng/ml and 0.001-35.65 ng/ml, respectively. In addition, the recovery rate of the two methods ranged from 93.5% to 112.0%, and the coefficient of variation (CV) was less than 10%. Therefore, the methods developed in this work have the merits of low cost, simple operation, and high sensitivity, which provide an effective analytical tool for BAC residue detection in feed samples.
杆菌肽锌（BAC）是一种多肽抗生素，因其具有促进动物生长的能力而被用作饲料添加剂。然而，BAC 的滥用会对食品安全造成极大威胁。因此，迫切需要开发一种快速、灵敏的检测方法。本研究获得了一种针对 BAC 的单克隆抗体（mAb），该 mAb 具有优异的灵敏度和特异性。首次将量子点（QDs）与制备的针对 BAC 的 mAb 和兔抗鼠抗体偶联，制备了直接和间接竞争荧光酶联免疫吸附测定法（dc-FLISA 和 ic-FLISA）来检测 BAC。dc-FLISA 和 ic-FLISA 的 IC 分别为 0.28 ng/ml 和 0.17 ng/ml，检测限分别为 0.0016 ng/ml 和 0.001 ng/ml，检测范围分别为 0.0016-46.50 ng/ml 和 0.001-35.65 ng/ml。此外，两种方法的回收率在 93.5%至 112.0%之间，变异系数（CV）小于 10%。因此，本工作中开发的方法具有成本低、操作简单、灵敏度高的优点，为饲料样品中 BAC 残留的检测提供了有效的分析工具。
