人胃蛋白酶1、3和5对卵清蛋白的消化作用。
Ovalbumin digestion by human pepsins 1, 3 and 5.
作者信息
Walker V, Taylor W H
出版信息
Biochem J. 1978 Nov 15;176(2):429-32. doi: 10.1042/bj1760429.
Abstract
- Of the three major human pepsins, pepsin 1 has greater proteolytic activity towards ovalbumin than has pepsin 3. Pepsin 5 has low activity towards this substrate. 2. Proteolytic pH-activity curves show only on pH maximum, about pH 1.4 for pepsin 1, pH 1.4--1.5 for pepsin 3 and pH 1.2--1.4 for pepsin 5. The curve for pepsin 3 has a shoulder between pH 2.4 and 3.4. 3. The rate of digestion of ovalbumin by pepsin 1 is approximately three times slower than are those of bovine haemoglobin or human globin. 4. The results suggest that there may be a physiological advantage in having more than one pepsin.
摘要
- 在三种主要的人类胃蛋白酶中，胃蛋白酶1对卵清蛋白的蛋白水解活性比胃蛋白酶3更高。胃蛋白酶5对该底物的活性较低。2. 蛋白水解pH活性曲线仅显示一个pH最大值，胃蛋白酶1约为pH 1.4，胃蛋白酶3为pH 1.4 - 1.5，胃蛋白酶5为pH 1.2 - 1.4。胃蛋白酶3 的曲线在pH 2.4至3.4之间有一个肩峰。3. 胃蛋白酶1对卵清蛋白的消化速率比牛血红蛋白或人球蛋白的消化速率慢约三倍。4. 结果表明，拥有不止一种胃蛋白酶可能具有生理优势。
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