Department of Thoracic Surgery, Tokyo Medical University, Tokyo, Japan.
Department of Anatomical Pathology, Tokyo Medical University, Tokyo, Japan.
PLoS One. 2021 Mar 5;16(3):e0248206. doi: 10.1371/journal.pone.0248206. eCollection 2021.
Distinguishing lung squamous cell carcinoma (LSQCC) from a solitary metastatic lung tumor (MSQCC) from head and neck squamous cell carcinoma (HNSQCC) presents a difficult diagnostic challenge even after detailed pathological assessment. Treatment options and estimated survival outcomes after pulmonary resection differ between patients with LSQCC and MSQCC. This study aimed to investigate whether microRNA (miRNA) profiling by RNA sequencing of HNSQCC, MSQCC, and LSQCC was useful for differential diagnosis of MSQCC and LSQCC. RNA sequencing was performed to identify bioinformatically significant miRNAs from a formalin-fixed paraffin-embedded (FFPE) block from a derivation set. MiRNA levels were confirmed by validation sets using FFPE samples and serum extracellular vesicles from patients. Step-wise discriminant analysis and canonical discriminant analysis identified 13 miRNAs by which the different expression patterns of LSQCC, MSQCC, and HNSQCC groups were demonstrated. Six miRNAs (miR-10a/28/141/320b/3120) were assessed in validation sets, and 4 miRNAs (miR-10a/28/141/3120) were significantly upregulated in LSQCCs compared with MSQCCs and HNSQCCs. Serum extracellular vesicles from LSQCC patients demonstrated significantly elevated miR-10a (p = .042), miR-28 (p = .041), and miR-3120 (p = .047) levels compared with those from MSQCC patients. RNA sequencing is useful for differential diagnosis of LSQCC and MSQCC, and the expression level of miR-10a, miR-28, and miR-3120 in serum extracellular vesicles are promising noninvasive tools for this purpose.
鉴别肺鳞状细胞癌 (LSQCC) 与来自头颈部鳞状细胞癌 (HNSQCC) 的孤立性转移性肺肿瘤 (MSQCC) 即使在详细的病理评估后也具有挑战性。肺切除术后，LSQCC 和 MSQCC 患者的治疗选择和估计生存结果不同。本研究旨在探讨 HNSQCC、MSQCC 和 LSQCC 的 RNA 测序微 RNA (miRNA) 谱分析是否有助于 MSQCC 和 LSQCC 的鉴别诊断。通过对衍生集福尔马林固定石蜡包埋 (FFPE) 块进行 RNA 测序，从生物信息学上鉴定出有意义的 miRNA。使用 FFPE 样本和患者血清细胞外囊泡在验证集中验证 miRNA 水平。逐步判别分析和典型判别分析确定了 13 个 miRNA，通过这些 miRNA 可以展示 LSQCC、MSQCC 和 HNSQCC 组的不同表达模式。在验证集中评估了 6 个 miRNA（miR-10a/28/141/320b/3120），与 MSQCC 和 HNSQCC 相比，LSQCC 中 4 个 miRNA（miR-10a/28/141/3120）显著上调。与 MSQCC 患者相比，LSQCC 患者的血清细胞外囊泡中 miR-10a（p =.042）、miR-28（p =.041）和 miR-3120（p =.047）水平显著升高。RNA 测序可用于 LSQCC 和 MSQCC 的鉴别诊断，血清细胞外囊泡中 miR-10a、miR-28 和 miR-3120 的表达水平是该目的有前途的非侵入性工具。
