Desantis Salvatore, Galosi Livio, Santamaria Nicoletta, Roncarati Alessandra, Biagini Lucia, Rossi Giacomo
Department of Emergency and Organ Transplantation (DETO), University of Bari Aldo Moro, S.P. 62 per Casamassima Km 3, 70010 Valenzano (Bari), Italy.
School of Biosciences and Veterinary medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica (M.C.), Italy.
Animals (Basel). 2021 Feb 13;11(2):495. doi: 10.3390/ani11020495.
Probiotics have become highly recognized as supplements for poultry.Since gut health can be considered synonymous withanimal health, the effects of probiotic Slab51 on the morphology and the glycan composition of guineafowlintestine were examined. The probiotics were added in drinking water (2 × 10 UFC/L) throughout the grow-out cycle.Birds were individually weighed andslaughtered after four months. Samples from the duodenum, ileum and caecum were collected and processed for morphological, morphometric, conventional and lectin glycohistochemical studies.The results were analyzed for statistical significance by Student's test. Compared with control samples, probiotic group revealed (1) significant increase in villus height ( < 0.001 in duodenum and ileum; < 0.05 in caecum), crypt depth ( < 0.001 in duodenum and caecum; < 0.05 in ileum) and goblet cells (GCs) per villus ( < 0.001) in all investigated tracts; (2) increase in galactoseβl,3N-acetylgalacyosamine(Galβl,3GalNAc)terminating O-glycans and αl,2-fucosylated glycans secretory GCs in the duodenum; (3) increase in α2,6-sialoglycans and high-mannose N-linked glycans secretory GCs but reduction in GCs-secreting sulfoglycans in the ileum; (4) increase in Galβl,3GalNAc and high-mannose N-linked glycans secretory GCs and decrease in GCs-producing sulfomucins in the caecum; (5) increase in the numbers of crypt cells containing sulfate and non-sulfated acidic glycans. Overall, dietary Slab51 induces morphological and region-specific changes in glycoprotein composition of guinea fowl intestine, promoting gut health.
益生菌作为家禽的补充剂已得到高度认可。由于肠道健康可被视为动物健康的同义词，因此研究了益生菌Slab51对珍珠鸡肠道形态和聚糖组成的影响。在整个生长周期中，将益生菌添加到饮用水中（2×10 UFC/L）。四个月后对鸡进行个体称重并宰杀。收集十二指肠、回肠和盲肠的样本，并进行形态学、形态计量学、常规和凝集素糖组化研究。通过学生t检验分析结果的统计学意义。与对照样本相比，益生菌组显示：（1）在所有研究肠道中，绒毛高度显著增加（十二指肠和回肠中P<0.001；盲肠中P<0.05）、隐窝深度显著增加（十二指肠和盲肠中P<0.001；回肠中P<0.05）以及每个绒毛的杯状细胞（GCs）数量显著增加（P<0.001）；（2）十二指肠中分泌GCs的半乳糖β1,3N-乙酰半乳糖胺（Galβ1,3GalNAc）末端O-聚糖和α1,2-岩藻糖基化聚糖增加；（3）回肠中分泌GCs的α2,6-唾液酸聚糖和高甘露糖N-连接聚糖增加，但分泌硫酸化聚糖的GCs减少；（4）盲肠中分泌GCs的Galβ1,3GalNAc和高甘露糖N-连接聚糖增加，产生硫黏液素的GCs减少；（5）含有硫酸盐和非硫酸盐酸性聚糖的隐窝细胞数量增加。总体而言，日粮中的Slab51诱导珍珠鸡肠道糖蛋白组成发生形态学和区域特异性变化，促进肠道健康。
