Trindade Rafaela, Almeida Laís, Xavier Luciana, Andrade Eloisa Helena, Maia José Guilherme, Mello Andréa, Setzer William N, Ramos Alessandra, da Silva Joyce Kelly R
Programa de Pós-Graduação em Biotecnologia, Universidade Federal do Pará, Belém 66075-110, Brazil.
Laboratório de Biotecnologia de Enzimas e Biotransformações, Universidade Federal do Pará, Belém 66075-110, Brazil.
Microorganisms. 2021 Feb 25;9(3):484. doi: 10.3390/microorganisms9030484.
To elucidate defense mechanisms of against fusariosis, an experiment based on co-inoculation of arbuscular mycorrhizal fungi (AMF) and f. sp. was performed. Variations in secondary metabolism in plants infected with f. sp. (FUS) and co-inoculated with AMFs and (AMF + FUS) were monitored at 7- and 21-days post inoculations (dpi). The pathogen induced a decrease in oxygenated sesquiterpenes (82.0-77.4%), and changes in the concentrations of the main compounds, α-muurolene, α-muurolol, and 2-hexenal in the leaves. It was observed that the concentration of 2-hexenal decreased at 7 dpi, α-muurolene decreased at 21 dpi, and α-muurolol increased at 21 dpi. There was a prevalence of sesquiterpene and monoterpene hydrocarbons in the roots, such as β-caryophyllene, δ-elemene, and limonene. The infection and co-inoculation induced greater production of phenolics in the roots at 7 dpi. The enzymatic activity of phenylalanine ammonia-lyase decreased in the leaves at 21 dpi and in the roots on both days, while the lipoxygenase activity decreased only in the roots at 21 dpi. The results demonstrated that co-inoculation with AMFs and induces changes in the defense metabolism of , but it is not efficient in the biocontrol of fusariosis during the evaluated period.
为阐明[植物名称]对镰刀菌病的防御机制，进行了一项基于丛枝菌根真菌（AMF）与[病原菌名称]f. sp. 共同接种的实验。在接种后7天和21天监测感染[病原菌名称]f. sp. （FUS）以及与AMF共同接种（AMF + FUS）的植物中次生代谢的变化。病原菌导致氧化倍半萜减少（82.0 - 77.4%），并使叶片中主要化合物α-穆罗勒烯、α-穆罗勒醇和2-己烯醛的浓度发生变化。观察到2-己烯醛浓度在接种后7天下降，α-穆罗勒烯在21天下降，α-穆罗勒醇在21天增加。根部存在倍半萜和单萜烃类物质，如β-石竹烯、δ-榄香烯和柠檬烯。感染和共同接种在接种后7天诱导根部产生更多酚类物质。苯丙氨酸解氨酶的酶活性在接种后21天在叶片中以及在两天内根部均下降，而脂氧合酶活性仅在接种后21天在根部下降。结果表明，与AMF共同接种会诱导[植物名称]防御代谢的变化，但在评估期间对镰刀菌病的生物防治并不有效。
