Rubí-Sans Gerard, Cano-Torres Irene, Pérez-Amodio Soledad, Blanco-Fernandez Barbara, Mateos-Timoneda Miguel A, Engel Elisabeth
Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain.
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28040 Madrid, Spain.
Biomedicines. 2021 Feb 25;9(3):232. doi: 10.3390/biomedicines9030232.
Tissue engineering and regenerative medicine approaches use biomaterials in combination with cells to regenerate lost functions of tissues and organs to prevent organ transplantation. However, most of the current strategies fail in mimicking the tissue's extracellular matrix properties. In order to mimic native tissue conditions, we developed cell-derived matrix (CDM) microtissues (MT). Our methodology uses poly-lactic acid (PLA) and Cultispher S microcarriers' (MCs') as scaffold templates, which are seeded with rat bone marrow mesenchymal stem cells (rBM-MSCs). The scaffold template allows cells to generate an extracellular matrix, which is then extracted for downstream use. The newly formed CDM provides cells with a complex physical (MT architecture) and biochemical (deposited ECM proteins) environment, also showing spontaneous angiogenic potential. Our results suggest that MTs generated from the combination of these two MCs (mixed MTs) are excellent candidates for tissue vascularization. Overall, this study provides a methodology for in-house fabrication of microtissues with angiogenic potential for downstream use in various tissue regenerative strategies.
组织工程和再生医学方法利用生物材料与细胞相结合，以恢复组织和器官丧失的功能，从而避免器官移植。然而，目前大多数策略在模拟组织的细胞外基质特性方面均告失败。为了模拟天然组织条件，我们开发了细胞衍生基质（CDM）微组织（MT）。我们的方法使用聚乳酸（PLA）和Cultispher S微载体（MC）作为支架模板，接种大鼠骨髓间充质干细胞（rBM-MSC）。支架模板使细胞能够生成细胞外基质，随后将其提取以供下游使用。新形成的CDM为细胞提供了一个复杂的物理（MT结构）和生化（沉积的细胞外基质蛋白）环境，还显示出自发血管生成潜力。我们的结果表明，由这两种MC组合产生的MT（混合MT）是组织血管化的极佳候选者。总体而言，本研究提供了一种在内部制造具有血管生成潜力的微组织的方法，以供在各种组织再生策略中进行下游应用。
