Bertuso Paula de Camargo, Mayer Débora M Drappé, Nitschke Marcia
Interunits Graduate Program in Bioengineering (EESC/FMRP/IQSC), University of São Paulo, Trabalhador São-carlense Av., 400, São Carlos, SP 13566-590, Brazil.
São Carlos Institute of Chemistry (IQSC), University of São Paulo, Trabalhador São-carlense Av., 400, P.O. Box 780, São Carlos, SP 13560-970, Brazil.
Foods. 2021 Feb 19;10(2):455. doi: 10.3390/foods10020455.
Foodborne diseases (FBD) are a great problem worldwide, leading millions of people to seek medical help and to significant economic losses for industry. Among the agents implicated in FDB is , a Gram-positive, toxigenic and endospore-forming bacterium. In this study, rhamnolipid (RL) biosurfactant, celery oleoresin (OR) and limonene (LN) were evaluated as bio-based alternatives for controlling the growth of vegetative cells and endospores of . To address their antimicrobial activity, the compounds were tested separately and in combination. Results demonstrate that, when combined with RL, both OR and LN have lower minimal inhibitory concentration (MIC) values and increased endospore inhibition potential. A percentage of endospore inhibition from 73% to 98%, corresponding to a 2.8-3.6 log reduction in spore outgrowth, was observed. RL inhibited growth and endospore germination and potentially enhanced the antimicrobial efficacy of the natural hydrophobic compounds tested.
食源性疾病在全球范围内是一个重大问题,导致数百万人寻求医疗帮助,并给相关产业带来巨大经济损失。在引发食源性疾病的病原体中,有一种革兰氏阳性、产毒素且形成芽孢的细菌。在本研究中,鼠李糖脂(RL)生物表面活性剂、芹菜油树脂(OR)和柠檬烯(LN)被评估为控制该细菌营养细胞和芽孢生长的生物基替代物。为了研究它们的抗菌活性,分别对这些化合物及其组合进行了测试。结果表明,与RL联合使用时,OR和LN的最低抑菌浓度(MIC)值均较低,且芽孢抑制潜力增强。观察到芽孢抑制率从73%到98%,相当于芽孢生长减少2.8 - 3.6个对数。RL抑制了该细菌的生长和芽孢萌发,并可能增强了所测试天然疏水化合物的抗菌效果。