Zhang Yi, Jiang Wei, Feng Dezhi, Wang Chenguang, Xu Yi, Shan Yufeng, Wang Jianlu, Yin Ziwei, Deng Huiyong, Mi Xianqiang, Dai Ning
State Key Laboratory of Infrared Physics, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China.
School of Electronic Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
Nanomaterials (Basel). 2021 Feb 21;11(2):545. doi: 10.3390/nano11020545.
2D molybdenum disulfide (MoS)-based thin film transistors are widely used in biosensing, and many efforts have been made to improve the detection limit and linear range. However, in addition to the complexity of device technology and biological modification, the compatibility of the physical device with biological solutions and device reusability have rarely been considered. Herein, we designed and synthesized an array of MoS by employing a simple-patterned chemical vapor deposition growth method and meanwhile exploited a one-step biomodification in a sensing pad based on DNA tetrahedron probes to form a bio-separated sensing part. This solves the signal interference, solution erosion, and instability of semiconductor-based biosensors after contacting biological solutions, and also allows physical devices to be reused. Furthermore, the gate-free detection structure that we first proposed for DNA (BRCA1) detection demonstrates ultrasensitive detection over a broad range of 1 fM to 1 μM with a good linear response of R = 0.98. Our findings provide a practical solution for high-performance, low-cost, biocompatible, reusable, and bio-separated biosensor platforms.
基于二维二硫化钼（MoS）的薄膜晶体管在生物传感中被广泛应用，人们已做出诸多努力来提高检测限和线性范围。然而，除了器件技术和生物修饰的复杂性外，物理器件与生物溶液的兼容性以及器件的可重复使用性很少被考虑。在此，我们采用简单图案化化学气相沉积生长方法设计并合成了一系列MoS，同时基于DNA四面体探针在传感垫中开发了一步生物修饰，以形成生物分离的传感部分。这解决了基于半导体的生物传感器接触生物溶液后的信号干扰、溶液侵蚀和不稳定性问题，还使物理器件能够重复使用。此外，我们首次提出的用于DNA（BRCA1）检测的无栅极检测结构在1 fM至1 μM的宽范围内展示了超灵敏检测，线性响应良好，R = 0.98。我们的研究结果为高性能、低成本、生物相容、可重复使用和生物分离的生物传感器平台提供了一个切实可行的解决方案。
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