Song Pengfei, Ou Pengfei, Wang Yongjie, Yuan Hang, Duan Sixuan, Chen Longyan, Fu Hao, Song Jun, Liu Xinyu
Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, Ontario, M5S 3G8, Canada; Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, Montreal, Quebec, H3A 0C3, Canada; School of Advanced Technology, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou, 215000, China.
Department of Mining and Materials Engineering, McGill University, 3610 Rue University, Montreal, Quebec, H3A 0C5, Canada.
Anal Chim Acta. 2023 Apr 29;1252:341036. doi: 10.1016/j.aca.2023.341036. Epub 2023 Mar 3.
Molybdenum disulfide (MoS) nanolayers are one of the most promising two-dimensional (2D) nanomaterials for constructing next-generation field-effect transistor (FET) biosensors. In this article, we report an ultrasensitive FET biosensor that integrates a novel format of 2D MoS, vertically-aligned MoS nanolayers (VAMNs), as the channel material for label-free detection of the prostate-specific antigen (PSA). The developed VAMNs-based FET biosensor shows two distinctive advantages. First, the VAMNs can be facilely grown using the conventional chemical vapor deposition (CVD) method, permitting easy fabrication and potential mass device production. Second, the unique advantage of the VAMNs for biosensor development lies in its abundant surface-exposed active edge sites that possess a high binding affinity with thiol-based linkers, which overcomes the challenge of molecule functionalization on the conventional planar MoS nanolayers. The high binding affinity between 11-mercaptoundecanoic acid and the VAMNs was demonstrated through experimental surface characterization and theoretical calculations via density functional theory. The FET biosensor allows rapid (within 20 min) and ultrasensitive PSA detection in human serum with simple operations (limit of detection: 800 fg mL). This FET biosensor offers excellent features such as ultrahigh sensitivity, ease of fabrication, and short assay time, and thereby possesses significant potential for early-stage diagnosis of life-threatening diseases.
二硫化钼（MoS）纳米层是构建下一代场效应晶体管（FET）生物传感器最有前景的二维（2D）纳米材料之一。在本文中，我们报道了一种超灵敏FET生物传感器，该传感器集成了一种新型二维MoS结构，即垂直排列的MoS纳米层（VAMNs），作为用于无标记检测前列腺特异性抗原（PSA）的通道材料。所开发的基于VAMNs的FET生物传感器具有两个显著优点。首先，VAMNs可以使用传统的化学气相沉积（CVD）方法轻松生长，便于制造和潜在的大规模器件生产。其次，VAMNs在生物传感器开发方面的独特优势在于其大量表面暴露的活性边缘位点，这些位点与基于硫醇的连接体具有高结合亲和力，克服了传统平面MoS纳米层上分子功能化的挑战。通过实验表面表征和基于密度泛函理论的理论计算，证明了11 - 巯基十一烷酸与VAMNs之间的高结合亲和力。该FET生物传感器能够在人血清中快速（20分钟内）且超灵敏地检测PSA，操作简单（检测限：800 fg/mL）。这种FET生物传感器具有超高灵敏度、易于制造和检测时间短等优异特性，因此在危及生命疾病的早期诊断方面具有巨大潜力。
