Chu A, Schroepfer G J
Department of Biochemistry, Rice University, Houston, TX 77251.
J Lipid Res. 1988 Feb;29(2):235-9.
A relatively simple and rapid method was required for the separation of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one, a potent inhibitor of sterol synthesis, from its major metabolites. Conditions have been determined which permit the resolution of the 15-ketosterol and cholesterol and fatty acid esters of the two sterols by reverse phase high performance chromatography. This methodology also permits the resolution of the major esters of the 15-ketosterol and of cholesterol.
需要一种相对简单快速的方法，从其主要代谢产物中分离出5α-胆甾-8(14)-烯-3β-醇-15-酮，一种有效的甾醇合成抑制剂。已确定了相关条件，可通过反相高效液相色谱法分离15-酮甾醇以及两种甾醇的胆固醇和脂肪酸酯。该方法还可分离15-酮甾醇和胆固醇的主要酯类。
