Salimi Mohsen, Sun Brigitta R, Tabunag Jenny Syl, Li Jianxiong, Yu Hua-Zhong
Department of Chemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.
Faculty of Chemistry, Iran University of Science and Technology, Tehran 16846-11367, Iran.
Micromachines (Basel). 2021 Feb 28;12(3):246. doi: 10.3390/mi12030246.
Anthocyanins are antioxidant and anti-inflammatory ingredients in various fruit beverages, for which their conservation and quantitation are important for the food industry. In this paper, we report a simple, portable device for accurate on-site determination of total monomeric anthocyanins in fruit beverages employing a Wi-Fi scanner coupled with a flexible microchip and a free mobile app. The detection principle is based on the pH-induced colorimetric reactions of anthocyanins performed in a specially designed microchip and validated with standard spectrophotometric measurements. The microchip with multiple testing vials was prepared with the benchtop molding method with a common PDMS elastomer and a transparency film; the photo of the scanned microchip is wirelessly sent to a smartphone and the RGB values of individual reaction vials in the microchip are analyzed with a free mobile app to determine the corresponding concentrations. It was demonstrated that the quantitation performance of this POCT device is comparable with conventional spectrophotometry in the determination of total anthocyanins in both standard solutions and fruit beverages.
花青素是各种水果饮料中的抗氧化和抗炎成分，其保存和定量对食品工业很重要。在本文中，我们报告了一种简单、便携的设备，该设备利用Wi-Fi扫描仪、柔性微芯片和免费移动应用程序，可在现场准确测定水果饮料中的总单体花青素。检测原理基于在专门设计的微芯片中进行的花青素pH诱导比色反应，并通过标准分光光度测量进行验证。带有多个测试小瓶的微芯片采用台式成型方法，使用普通的聚二甲基硅氧烷（PDMS）弹性体和透明薄膜制备；扫描后的微芯片照片被无线发送到智能手机，微芯片中各个反应小瓶的RGB值通过免费移动应用程序进行分析，以确定相应的浓度。结果表明，在测定标准溶液和水果饮料中的总花青素时，这种即时检测（POCT）设备的定量性能与传统分光光度法相当。
