Giacobbo Franciele C N, Eyng Cinthia, Nunes Ricardo V, Souza Cleison de, Teixeira Levy V, Pilla Rachel, Suchodolski Jan S, Bortoluzzi Cristiano
Ciências Agrárias, Universidade Estadual do Oeste do Paraná, Mal. C. Rondon 85960-000, Brazil.
DSM Nutritional Products, São Paulo 04551-065, Brazil.
Animals (Basel). 2021 Feb 28;11(3):643. doi: 10.3390/ani11030643.
We evaluated the influence of enzymatic supplementation on the growth performance and cecal microbiota of broilers. A total of 2160 1-day-old male chicks were used in a 3 × 2 × 2 factorial arrangement (three corn hybrids, two drying temperatures -80 and 110 °C, with or without the inclusion of an enzymatic blend (amylase, xylanase, and protease) (20 birds/pen, = 9). For all performance and digestibility parameters, we observed, in general, isolated effects of the corn hybrids and drying temperature. Birds that received the enzymatic blend in the diet showed better weight gain from 1 to 21 days (d) and better digestibility coefficients of nutrients at 42 d. Birds fed diets with corn dried at 80 °C showed a better feed conversion ratio from 1 to 42 d. At 21 d of age, enzymatic supplementation had positive effects on jejunum morphology. Enzyme supplementation increased the abundance of the phylum Tenericutes, class Bacilli and Mollicutes, reduced Clostridia, and increased the abundances of the families Lactobacillaceae, Anaeroplasmataceae, and O_RF39;F. In conclusion, the addition of amylase, xylanase, and protease led to a better nutrient digestibility, performance, and intestinal morphology. In addition, enzyme supplementation changed the diversity, composition, and predicted function of the cecal microbiota at d 21.
我们评估了酶制剂添加对肉鸡生长性能和盲肠微生物群的影响。总共2160只1日龄雄性雏鸡采用3×2×2析因设计(三种玉米杂交种、两种干燥温度——80℃和110℃,添加或不添加酶制剂组合(淀粉酶、木聚糖酶和蛋白酶))(每栏20只鸡,n = 9)。对于所有性能和消化率参数,我们总体上观察到玉米杂交种和干燥温度的单独效应。日粮中添加酶制剂组合的鸡在1至21日龄时体重增加更好,在42日龄时养分消化率系数更高。饲喂80℃干燥玉米日粮的鸡在1至42日龄时饲料转化率更好。在21日龄时,添加酶制剂对空肠形态有积极影响。添加酶制剂增加了柔膜菌门、芽孢杆菌纲和柔膜菌纲的丰度,减少了梭菌,增加了乳杆菌科、无胆甾原体科和O_RF39;F科的丰度。总之,添加淀粉酶、木聚糖酶和蛋白酶可提高养分消化率、性能和肠道形态。此外,添加酶制剂在21日龄时改变了盲肠微生物群的多样性、组成和预测功能。