Stefanello C, Vieira S L, Santiago G O, Kindlein L, Sorbara J O B, Cowieson A J
Departamento de Zootecnia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 7712, Porto Alegre, RS, Brazil, 91540-000.
Departamento de Zootecnia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 7712, Porto Alegre, RS, Brazil, 91540-000
Poult Sci. 2015 Oct;94(10):2472-9. doi: 10.3382/ps/pev244. Epub 2015 Aug 27.
A study was conducted to evaluate the effects of dietary α-amylase and β-xylanase supplementation of corn-soy diets, formulated with or without supplemental phytase, on growth performance, energy utilization, and starch digestibility in broiler chickens. A total of 336 slow-feathering, Cobb × Cobb 500 male broilers were randomly distributed to 6 treatments having 8 replicates of 7 birds each. Birds were fed a common starter diet to d 14 post-hatch (3,050 kcal/kg AMEn, 21.7% CP, 1.05% Ca, and 0.53% nPP). The experimental diets were provided afterwards until d 25. A 2 × 3 factorial arrangement of 2 control diets (basal = corn-soy diet without added phytase or PHY = corn-soy diet formulated with 1,000 phytase units/kg) and 3 carbohydrase supplementations (0, 80 kilo-Novo α-amylase units/kg, or 80 kilo-Novo α-amylase units/kg + 100 fungal β-xylanase units/kg) was used from d 14 to 25. Excreta were collected from 21 to 24 d and all birds were euthanized at 25 d for jejunum and ileum content collection. Samples of feed, excreta, and jejunal and ileal digesta were analyzed for determination of total tract retention and ileal apparent digestibility. No interactions between diet and carbohydrase were observed. Broilers fed diets formulated with phytase or supplemented with amylase + xylanase had higher BW gain (BWG) and lower FCR (P < 0.05) when compared with birds fed diets without carbohydrases. Relative to the basal diet, AMEn was increased (P < 0.01) by 70 kcal/kg and 99 kcal/kg when birds were fed the diet supplemented with amylase and amylase + xylanase, respectively. Starch digestibility in the jejunum and ileum was increased (P < 0.05) by 3.5% and 2.4%, respectively, when birds were fed the diet supplemented with amylase + xylanase. Results from this experiment show that corn-soy diets having phytase and supplemented with amylase and xylanase led to increased growth performance, AMEn, and starch digestibility in broilers. Furthermore, the efficacy of exogenous amylase and xylanase was independent of the presence of microbial phytase.
本研究旨在评估在添加或不添加植酸酶的玉米 - 大豆型日粮中添加α - 淀粉酶和β - 木聚糖酶对肉鸡生长性能、能量利用率和淀粉消化率的影响。总共336只生长缓慢的科宝×科宝500雄性肉鸡被随机分配到6种处理中,每种处理有8个重复,每个重复7只鸡。雏鸡在孵化后第14天前饲喂普通开食料(3,050千卡/千克表观代谢能、21.7%粗蛋白、1.05%钙和0.53%非植酸磷)。之后提供实验日粮直至第25天。从第14天到第25天采用2×3析因设计,即2种对照日粮(基础日粮 = 不添加植酸酶的玉米 - 大豆日粮或PHY = 添加1,000植酸酶单位/千克的玉米 - 大豆日粮)和3种碳水化合物酶添加水平(0、80千诺维信α - 淀粉酶单位/千克或80千诺维信α - 淀粉酶单位/千克 + 100真菌β - 木聚糖酶单位/千克)。在第21天至第24天收集排泄物,所有鸡在第25天安乐死以收集空肠和回肠内容物。分析饲料、排泄物以及空肠和回肠食糜样本以测定全肠道存留率和回肠表观消化率。未观察到日粮与碳水化合物酶之间的相互作用。与未添加碳水化合物酶的日粮组相比,饲喂添加植酸酶或添加淀粉酶 + 木聚糖酶日粮的肉鸡体重增加(BWG)更高且料重比(FCR)更低(P < 0.05)。相对于基础日粮,当肉鸡分别饲喂添加淀粉酶和淀粉酶 + 木聚糖酶的日粮时,表观代谢能分别提高了70千卡/千克和99千卡/千克(P < 0.01)。当肉鸡饲喂添加淀粉酶 + 木聚糖酶的日粮时,空肠和回肠中的淀粉消化率分别提高了3.5%和2.4%(P < 0.05)。本实验结果表明,添加植酸酶并补充淀粉酶和木聚糖酶的玉米 - 大豆型日粮可提高肉鸡的生长性能、表观代谢能和淀粉消化率。此外,外源淀粉酶和木聚糖酶的功效与微生物植酸酶的存在无关。