Sokaribo Akosiererem S, Perera Sumudu R, Sereggela Zoe, Krochak Ryan, Balezantis Lindsay R, Xing Xiaohui, Lam Shirley, Deck William, Attah-Poku Sam, Abbott Dennis Wade, Tamuly Shantanu, White Aaron P
Vaccine and Infectious Disease Organization-International Vaccine Centre, Saskatoon, SK S7N5E3, Canada.
Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK S7N5E5, Canada.
Vaccines (Basel). 2021 Feb 17;9(2):165. doi: 10.3390/vaccines9020165.
Non-typhoidal are a major cause of gastroenteritis worldwide, as well as causing bloodstream infections in sub-Saharan Africa with a high fatality rate. No vaccine is currently available for human use. Current vaccine development strategies are focused on capsular polysaccharides (CPS) present on the surface of non-typhoidal . This study aimed to boost the amount of CPS purified from for immunization trials. Random mutagenesis with Tn transposon increased the production of CPS colanic acid, by 10-fold compared to wildtype. Immunization with colanic acid or colanic acid conjugated to truncated glycoprotein D or inactivated diphtheria toxin did not induce a protective immune response in mice. However, immunization with Generalized Modules for Membrane Antigens (GMMAs) isolated from colanic acid overproducing isolates reduced colonization in mice. Our results support the development of a GMMA-CPS-based vaccine against non-typhoidal .
非伤寒型[细菌名称未给出]是全球肠胃炎的主要病因，同时也是撒哈拉以南非洲地区导致血流感染且致死率很高的病因。目前尚无用于人类的疫苗。当前的疫苗研发策略聚焦于非伤寒型[细菌名称未给出]表面存在的荚膜多糖（CPS）。本研究旨在提高从[细菌名称未给出]中纯化的用于免疫试验的CPS量。用Tn转座子进行随机诱变使CPS柯氏酸的产量比野生型增加了10倍。用柯氏酸或与截短的糖蛋白D或灭活的白喉毒素偶联的柯氏酸进行免疫，在小鼠中未诱导出保护性免疫反应。然而，用从柯氏酸高产菌株中分离的膜抗原通用模块（GMMAs）进行免疫可减少[细菌名称未给出]在小鼠中的定植。我们的结果支持开发一种基于GMMA-CPS的抗非伤寒型[细菌名称未给出]疫苗。
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