• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于通用多价大分子抗原（GMMA）的疫苗引发的针对鼠伤寒血清型系统性感染的长期抗菌免疫

Long-Term Anti-Bacterial Immunity against Systemic Infection by Serovar Typhimurium Elicited by a GMMA-Based Vaccine.

作者信息

Fiorino Fabio, Pettini Elena, Koeberling Oliver, Ciabattini Annalisa, Pozzi Gianni, Martin Laura B, Medaglini Donata

机构信息

Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, 53100 Siena, Italy.

GSK Vaccines Institute for Global Health S.r.l., 53100 Siena, Italy.

出版信息

Vaccines (Basel). 2021 May 12;9(5):495. doi: 10.3390/vaccines9050495.

DOI:10.3390/vaccines9050495
PMID:34065899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8150838/
Abstract

Typhimurium (STm) represents the most prevalent cause of invasive non-typhoidal Salmonella (iNTS) disease, and currently no licensed vaccine is available. In this work we characterized the long-term anti-bacterial immunity elicited by a STm vaccine based on Generalized Modules of Membrane Antigens (GMMA) delivering O:4,5 antigen, using a murine model of systemic infection. Subcutaneous immunization of mice with STmGMMA/Alhydrogel elicited rapid, high, and persistent antigen-specific serum IgG and IgM responses. The serum was bactericidal in vitro. O:4,5-specific IgG were also detected in fecal samples after immunization and positively correlated with IgG observed in intestinal washes. Long-lived plasma cells and O:4,5-specific memory B cells were detected in spleen and bone marrow. After systemic STm challenge, a significant reduction of bacterial load in blood, spleen, and liver, as well as a reduction of circulating neutrophils and G-CSF glycoprotein was observed in STmGMMA/Alhydrogel immunized mice compared to untreated animals. Taken together, these data support the development of a GMMA-based vaccine for prevention of iNTS disease.

摘要

鼠伤寒沙门氏菌（STm）是侵袭性非伤寒沙门氏菌（iNTS）疾病最常见的病因，目前尚无获批的疫苗。在本研究中，我们利用系统性感染的小鼠模型，对基于递送O:4,5抗原的膜抗原通用模块（GMMA）的STm疫苗引发的长期抗菌免疫进行了表征。用STmGMMA/氢氧化铝凝胶对小鼠进行皮下免疫，可引发快速、强烈且持久的抗原特异性血清IgG和IgM反应。该血清在体外具有杀菌作用。免疫后在粪便样本中也检测到了O:4,5特异性IgG，且与肠道灌洗液中观察到的IgG呈正相关。在脾脏和骨髓中检测到了长寿浆细胞和O:4,5特异性记忆B细胞。与未处理的动物相比，在全身性STm攻击后，STmGMMA/氢氧化铝凝胶免疫的小鼠血液、脾脏和肝脏中的细菌载量显著降低，循环中性粒细胞和G-CSF糖蛋白也有所减少。综上所述，这些数据支持开发一种基于GMMA的疫苗来预防iNTS疾病。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/3bb9813d9377/vaccines-09-00495-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/06348a0da864/vaccines-09-00495-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/3a059ebac897/vaccines-09-00495-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/086049ab6fce/vaccines-09-00495-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/237fe3ced265/vaccines-09-00495-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/c7b96f0dfd59/vaccines-09-00495-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/5b26058063fe/vaccines-09-00495-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/3bb9813d9377/vaccines-09-00495-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/06348a0da864/vaccines-09-00495-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/3a059ebac897/vaccines-09-00495-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/086049ab6fce/vaccines-09-00495-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/237fe3ced265/vaccines-09-00495-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/c7b96f0dfd59/vaccines-09-00495-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/5b26058063fe/vaccines-09-00495-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a74/8150838/3bb9813d9377/vaccines-09-00495-g007.jpg

相似文献

1
Long-Term Anti-Bacterial Immunity against Systemic Infection by Serovar Typhimurium Elicited by a GMMA-Based Vaccine.基于通用多价大分子抗原（GMMA）的疫苗引发的针对鼠伤寒血清型系统性感染的长期抗菌免疫
Vaccines (Basel). 2021 May 12;9(5):495. doi: 10.3390/vaccines9050495.
2
IgG Responses to Porins and Lipopolysaccharide within an Outer Membrane-Based Vaccine against Nontyphoidal Develop at Discordant Rates.针对非伤寒血清型的外膜疫苗中对孔蛋白和脂多糖的 IgG 应答以不同速率发展。
mBio. 2018 Mar 6;9(2):e02379-17. doi: 10.1128/mBio.02379-17.
3
Vaccine Study in Oxford (SALVO) trial: protocol for an observer-participant blind randomised placebo-controlled trial of the iNTS-GMMA vaccine within a European cohort.牛津疫苗研究（SALVO）试验：在欧洲队列中，对 iNTS-GMMA 疫苗进行观察者-参与者盲法随机安慰剂对照试验的方案。
BMJ Open. 2023 Nov 14;13(11):e072938. doi: 10.1136/bmjopen-2023-072938.
4
Characterization of O-antigen delivered by Generalized Modules for Membrane Antigens (GMMA) vaccine candidates against nontyphoidal Salmonella.针对非伤寒沙门氏菌的膜抗原通用模块（GMMA）候选疫苗所传递的O抗原的特性分析。
Vaccine. 2017 Jan 11;35(3):419-426. doi: 10.1016/j.vaccine.2016.11.089. Epub 2016 Dec 18.
5
Comparative immunogenicity and efficacy of equivalent outer membrane vesicle and glycoconjugate vaccines against nontyphoidal .比较无伤寒血清型的等效外膜囊泡和糖缀合物疫苗的免疫原性和疗效。
Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):10428-10433. doi: 10.1073/pnas.1807655115. Epub 2018 Sep 27.
6
decorations in play a key role in eliciting functional immune responses against heterologous serovars in animal models.在动物模型中，荚膜多糖在引发针对异源血清型的功能性免疫反应方面起着关键作用。
Front Cell Infect Microbiol. 2024 Feb 29;14:1347813. doi: 10.3389/fcimb.2024.1347813. eCollection 2024.
7
Immunogenicity of a Bivalent Adjuvanted Glycoconjugate Vaccine against Typhimurium and Enteritidis.一种针对鼠伤寒沙门氏菌和肠炎沙门氏菌的二价佐剂糖缀合物疫苗的免疫原性。
Front Immunol. 2017 Feb 27;8:168. doi: 10.3389/fimmu.2017.00168. eCollection 2017.
8
Generalized Modules for Membrane Antigens (GMMA), an outer membrane vesicle-based vaccine platform, for efficient viral antigen delivery.通用膜抗原模块（GMMA），一种基于外膜囊泡的疫苗平台，用于有效递呈病毒抗原。
J Extracell Vesicles. 2022 Nov;11(11):e12247. doi: 10.1002/jev2.12247.
9
Toll-Like Receptor Activation by Generalized Modules for Membrane Antigens from Lipid A Mutants of Salmonella enterica Serovars Typhimurium and Enteritidis.鼠伤寒沙门氏菌和肠炎沙门氏菌脂多糖A突变体膜抗原通用模块对Toll样受体的激活作用
Clin Vaccine Immunol. 2016 Apr 4;23(4):304-14. doi: 10.1128/CVI.00023-16. Print 2016 Apr.
10
GMMA and Glycoconjugate Approaches Compared in Mice for the Development of a Vaccine against Serotype 6.在小鼠中比较GMMA和糖缀合物方法以开发抗6型血清型疫苗
Vaccines (Basel). 2020 Apr 3;8(2):160. doi: 10.3390/vaccines8020160.

引用本文的文献

1
A novel GMMA-based gonococcal vaccine demonstrates functional immune responses in mice.一种新型的基于颗粒性脑膜炎球菌疫苗（GMMA）的淋球菌疫苗在小鼠中显示出功能性免疫反应。
NPJ Vaccines. 2025 Jul 5;10(1):146. doi: 10.1038/s41541-025-01190-1.
2
One-tube detection of Typhimurium using LAMP and CRISPR-Cas12b.利用环介导等温扩增技术（LAMP）和 CRISPR-Cas12b 进行单管检测鼠伤寒沙门氏菌。
Microbiol Spectr. 2024 Oct 3;12(10):e0127124. doi: 10.1128/spectrum.01271-24. Epub 2024 Aug 27.
3
Emerging Strategies against Non-Typhoidal : From Pathogenesis to Treatment.

本文引用的文献

1
Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity?初次免疫和加强免疫之间的间隔长短：是否会影响疫苗免疫原性？
Vaccines (Basel). 2021 Mar 20;9(3):289. doi: 10.3390/vaccines9030289.
2
Dissecting the contribution of O-Antigen and proteins to the immunogenicity of Shigella sonnei generalized modules for membrane antigens (GMMA).解析志贺氏菌血清型 5 型通用膜抗原（GMMA）O 抗原和蛋白对其免疫原性的贡献。
Sci Rep. 2021 Jan 13;11(1):906. doi: 10.1038/s41598-020-80421-y.
3
Editorial: Advanced Immunization Technologies for Next Generation Vaccines.
针对非伤寒型的新兴策略：从发病机制到治疗
Curr Issues Mol Biol. 2024 Jul 14;46(7):7447-7472. doi: 10.3390/cimb46070442.
4
Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens.外膜囊泡作为发现针对细菌病原体抗体的平台。
Appl Microbiol Biotechnol. 2024 Feb 24;108(1):232. doi: 10.1007/s00253-024-13033-5.
5
Characterization of Enzyme-Linked Immunosorbent Assay (ELISA) for Quantification of Antibodies against Typhimurium and Enteritidis O-Antigens in Human Sera.用于定量检测人血清中抗鼠伤寒沙门氏菌和肠炎沙门氏菌O抗原抗体的酶联免疫吸附测定（ELISA）的特性分析
BioTech (Basel). 2023 Aug 11;12(3):54. doi: 10.3390/biotech12030054.
6
Preclinical evaluation of OMVs as potential vaccine candidates against serovar Enteritidis infection.针对肠炎沙门氏菌血清型感染，作为潜在疫苗候选物的 OMVs 的临床前评估。
Front Cell Infect Microbiol. 2022 Oct 27;12:1037607. doi: 10.3389/fcimb.2022.1037607. eCollection 2022.
7
Immune Memory After Respiratory Infection With Is Revealed by Stimulation of Murine Splenocytes With Inactivated Pneumococcal Whole Cells: Evidence of Early Recall Responses by Transcriptomic Analysis.呼吸道感染后免疫记忆的揭示：通过用灭活肺炎球菌全细胞刺激小鼠脾细胞：转录组分析证据表明早期回忆反应。
Front Cell Infect Microbiol. 2022 Jun 20;12:869763. doi: 10.3389/fcimb.2022.869763. eCollection 2022.
8
Dissecting the Activation of Human Immune Response Induced by GMMA.解析 GMMA 诱导的人类免疫应答的激活
Front Cell Infect Microbiol. 2022 Feb 3;12:767153. doi: 10.3389/fcimb.2022.767153. eCollection 2022.
社论：下一代疫苗的先进免疫技术
Front Immunol. 2020 May 6;11:878. doi: 10.3389/fimmu.2020.00878. eCollection 2020.
4
The global burden of non-typhoidal salmonella invasive disease: a systematic analysis for the Global Burden of Disease Study 2017.非伤寒型沙门氏菌侵袭性疾病的全球负担：2017 年全球疾病负担研究的系统分析。
Lancet Infect Dis. 2019 Dec;19(12):1312-1324. doi: 10.1016/S1473-3099(19)30418-9. Epub 2019 Sep 24.
5
Emerging Technologies for Low-Cost, Rapid Vaccine Manufacture.低成本、快速疫苗生产的新兴技术。
Biotechnol J. 2019 Jul;14(7):1-2. doi: 10.1002/biot.201970055.
6
Booster Vaccination With GVGH 1790GAHB GMMA Vaccine Compared to Single Vaccination in Unvaccinated Healthy European Adults: Results From a Phase 1 Clinical Trial.GVGH 1790GAHB GMMA 疫苗加强接种与未接种健康欧洲成年人单剂接种的比较：来自一项 1 期临床试验的结果。
Front Immunol. 2019 Mar 8;10:335. doi: 10.3389/fimmu.2019.00335. eCollection 2019.
7
Invasive Nontyphoidal Disease in Africa.非洲侵袭性非伤寒疾病
EcoSal Plus. 2019 Jan;8(2). doi: 10.1128/ecosalplus.ESP-0007-2018.
8
Comparative immunogenicity and efficacy of equivalent outer membrane vesicle and glycoconjugate vaccines against nontyphoidal .比较无伤寒血清型的等效外膜囊泡和糖缀合物疫苗的免疫原性和疗效。
Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):10428-10433. doi: 10.1073/pnas.1807655115. Epub 2018 Sep 27.
9
The global burden and epidemiology of invasive non-typhoidal infections.侵袭性非伤寒型沙门氏菌感染的全球负担和流行病学。
Hum Vaccin Immunother. 2019;15(6):1421-1426. doi: 10.1080/21645515.2018.1504717. Epub 2018 Sep 5.
10
Role of a single noncoding nucleotide in the evolution of an epidemic African clade of .一个单核苷酸在. 的流行非洲支系进化中的作用
Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):E2614-E2623. doi: 10.1073/pnas.1714718115. Epub 2018 Feb 27.