School of Mechanical Engineering, Korea University, Seoul 02841, Korea.
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
Sensors (Basel). 2021 Feb 15;21(4):1366. doi: 10.3390/s21041366.
Blood plasma is a source of biomarkers in blood and a simple, fast, and easy extraction method is highly required for point-of-care testing (POCT) applications. This paper proposes a membrane filter integrated microfluidic device to extract blood plasma from whole blood, without any external instrumentation. A commercially available membrane filter was integrated with a newly designed dual-cover microfluidic device to avoid leakage of the extracted plasma and remaining blood cells. Nano-interstices installed on both sides of the microfluidic channels actively draw the extracted plasma from the membrane. The developed device successfully supplied 20 μL of extracted plasma with a high extraction yield (~45%) in 16 min.
血浆是血液中的生物标志物来源，对于即时检测（POCT）应用，需要一种简单、快速且易于提取的方法。本文提出了一种膜过滤集成微流控装置，可从全血中提取血浆，无需任何外部仪器。商用膜过滤器与新设计的双盖微流控装置集成在一起，以避免提取的血浆和残留血细胞的泄漏。安装在微流道两侧的纳米间隙主动从膜中提取提取的血浆。所开发的设备成功地在 16 分钟内提供了 20 μL 的提取血浆，提取率高（约 45%）。
