Kim Pilkee, Ong Eng Hui, Li King Ho Holden, Yoon Yong-Jin, Ng Sum Huan Gary, Puttachat Khuntontong
School of Mechanical and Aerospace Engineering, Nanyang Technological University , 50 Nanyang Ave., Singapore 639798.
Singapore Institute of Manufacturing Technology (SIMTech) , 71 Nanyang Dr., Singapore 638075.
Biomicrofluidics. 2016 Mar 17;10(2):024110. doi: 10.1063/1.4944587. eCollection 2016 Mar.
Blood plasma contains biomarkers and substances that indicate the physiological state of an organism, and it can be used to diagnose various diseases or body condition. To improve the accuracy of diagnostic test, it is required to obtain the high purity of blood plasma. This paper presents a low-cost, disposable microfluidics device for blood plasma extraction using magnetophoretic behaviors of blood cells. This device uses alternating magnetophoretic capture modes to trap and separate paramagnetic and diamagnetic cells away from blood plasma. The device system is composed of two parts, a disposable microfluidics chip and a non-disposable (reusable) magnetic field source. Such modularized device helps the structure of the disposable part dramatically simplified, which is beneficial for low-cost mass production. A series of numerical simulation and parametric study have been performed to describe the mechanism of blood cell separation in the microchannel, and the results are discussed. Furthermore, experimental feasibility test has been carried out in order to demonstrate the blood plasma extraction process of the proposed device. In this experiment, pure blood plasma has been successfully extracted with yield of 21.933% from 75 μl 1:10 dilution of deoxygenated blood.
血浆中含有指示生物体生理状态的生物标志物和物质，可用于诊断各种疾病或身体状况。为提高诊断测试的准确性，需要获得高纯度的血浆。本文提出了一种利用血细胞磁泳行为进行血浆提取的低成本一次性微流控装置。该装置采用交替磁泳捕获模式，将顺磁性和抗磁性细胞从血浆中捕获并分离出来。该装置系统由两部分组成，一个一次性微流控芯片和一个非一次性（可重复使用）的磁场源。这种模块化装置有助于大大简化一次性部件的结构，有利于低成本大规模生产。已进行了一系列数值模拟和参数研究来描述微通道中血细胞分离的机制，并对结果进行了讨论。此外，还进行了实验可行性测试，以证明所提出装置的血浆提取过程。在该实验中，从75μl 1:10稀释的脱氧血液中成功提取了纯血浆，提取率为21.933%。
