Xin Chen, Song Shaozhen, Wang Ningli, Wang Ruikang, Johnstone Murray
Beijing Tongren Eye Center, Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
Life (Basel). 2021 Feb 23;11(2):176. doi: 10.3390/life11020176.
To evaluate the change of biomechanical properties of the trabecular meshwork (TM) and configuration of collector channels (CC) by high-resolution optical coherence tomography (HR-OCT) induced by Schlemm's canal (SC) dilation.
The anterior segments of two human eyes were divided into four quadrants. One end of a specially designed cannula was placed in SC and the other end connected to a perfusion reservoir. HR-OCT provided three-dimensional (3D) volumetric and two-dimensional (2D) cross-sectional imaging permitting assessment of the biomechanical properties of the TM. A large fluid bolus was introduced into SC. Same-sample, pre and post deformation and disruption of SC and CC lumen areas were analyzed.
Morphologic 3D reconstructions documented pressure-dependent changes in lumen dimension of SC, CC, and circumferential intrascleral channels. 2D imaging established volumetric stress-strain curves (elastance curves) of the TM in quadrants. The curves of TM elastance shift to the right with an increase in pressure-dependent steady-state SC area. After a bolus disruption, the SC area increased, while the CC area decreased.
Our experimental setup permits the study of the biomechanical properties of TM by examining elastance, which differs segmentally and is altered by mechanical expansion of SC by a fluid bolus. The study may shed light on mechanisms of intraocular pressure control of some glaucoma surgery.
通过高分辨率光学相干断层扫描（HR-OCT）评估施莱姆管（SC）扩张引起的小梁网（TM）生物力学特性变化及集液管（CC）形态。
将两只人眼的眼前节分为四个象限。将一根特制套管的一端置于SC内，另一端连接至灌注储液器。HR-OCT提供三维（3D）容积成像和二维（2D）断层成像，用于评估TM的生物力学特性。向SC内注入大量液体。分析同一标本在SC和CC管腔区域变形和破坏前后的情况。
形态学3D重建记录了SC、CC和巩膜内周向通道管腔尺寸的压力依赖性变化。2D成像建立了各象限TM的容积应力-应变曲线（弹性曲线）。随着压力依赖性稳态SC面积增加，TM弹性曲线右移。注入液体破坏后，SC面积增加，而CC面积减小。
我们的实验装置通过检测弹性来研究TM的生物力学特性，弹性在各节段有所不同，并因液体注入导致的SC机械扩张而改变。该研究可能有助于揭示某些青光眼手术眼压控制的机制。
