Noteborn H P, Graf M V, Ernst A, Schoenenberger G A, Weusten J A, Ebels I, Salemink C A
Department of Organic Chemistry, University of Utrecht, The Netherlands.
J Pineal Res. 1988;5(2):161-77. doi: 10.1111/j.1600-079x.1988.tb00779.x.
The nonapeptide delta-sleep-inducing peptide (DSIP) has been isolated from venous blood of rabbits induced to sleep. Numerous reports have described sleep as well as extra-sleep effects. Radiochemical and immunochemical data suggest a relationship of DSIP with the pineal gland supported by interactions of this peptide with pineal functions such as the serotonin N-acetyltransferase activity. In order to demonstrate the natural occurrence of DSIP-like material associated with high Mr proteins in the ovine pineal, organs were water-extracted and fractionated by ultrafiltration and gel filtration. Radioimmunoassay (RIA) for DSIP-like fragments of the fractions revealed considerable amounts of pineal DSIP-like immunoreactivity (DSIP-LI) apparently existing in small as well as large molecular forms. Acidification of large DSIP-LI forms resulted in the elution from Sephadex G-50 of Mr less than or equal to 1,000 DSIP-like material. This free DSIP-LI form coeluted with the synthetic DSIP nonapeptide from microBondapak C18 on high-performance liquid chromatography. The results, therefore, appear to indicate the presence of a (biospecific) noncovalent intermolecular interaction of DSIP (1-9) with proteins (Mr greater than or equal to 10,000) of the ovine pineal gland.
九肽δ-睡眠诱导肽（DSIP）已从诱导睡眠的兔静脉血中分离出来。许多报告描述了其睡眠以及睡眠外的作用。放射化学和免疫化学数据表明DSIP与松果体存在关联，这种关联得到了该肽与松果体功能（如血清素N-乙酰转移酶活性）相互作用的支持。为了证明绵羊松果体中存在与高分子量蛋白质相关的类DSIP物质，对器官进行水提取，然后通过超滤和凝胶过滤进行分级分离。对各分级部分的类DSIP片段进行放射免疫分析（RIA），结果显示松果体中存在大量的类DSIP免疫反应性物质（DSIP-LI），这些物质显然以小分子和大分子形式存在。大分子DSIP-LI形式经酸化后，可从Sephadex G-50洗脱下来分子量小于或等于1000的类DSIP物质。在高效液相色谱上，这种游离的DSIP-LI形式与合成的DSIP九肽在microBondapak C18上共洗脱。因此，这些结果似乎表明绵羊松果体中的DSIP（1-9）与蛋白质（分子量大于或等于10000）之间存在（生物特异性）非共价分子间相互作用。
