Guerrero Carlos, Collado-Boira Eladio, Martinez-Navarro Ignacio, Hernando Barbara, Hernando Carlos, Balino Pablo, Muriach María
Department of Medicine, Jaume I University, 12001 Castellon, Spain.
Faculty of Health Sciences, Jaume I University, 12001 Castellon, Spain.
Antioxidants (Basel). 2021 Feb 27;10(3):355. doi: 10.3390/antiox10030355.
Oxidative stress has been widely studied in association to ultra-endurance sports. Although it is clearly demonstrated the increase in reactive oxygen species and free radicals after these extreme endurance exercises, the effects on the antioxidant defenses and the oxidative damage to macromolecules, remain to be fully clarified. Therefore, the aim of this study was to elucidate the impact of an ultramarathon race on the plasma markers of oxidative stress of 32 runners and their post-race recovery, with especial focused on sex and age effect. For this purpose, the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase (GR) activity, as well as the lipid peroxidation product malondialdehyde (MDA) and the carbonyl groups (CG) content were measured before the race, in the finish line and 24 and 48 h after the race. We have reported an increase of the oxidative damage to lipids and proteins (MDA and CG) after the race and 48 h later. Moreover, there was an increase of the GR activity after the race. No changes were observed in runners' plasma GPx activity throughout the study. Finally, we have observed sex and age differences regarding damage to macromolecules, but no differences were found regarding the antioxidant enzymes measured. Our results suggest that several basal plasma markers of oxidative stress might be related to the extent of muscle damage after an ultraendurance race and also might affect the muscle strength evolution.
氧化应激已被广泛研究与超长耐力运动的关联。尽管在这些极端耐力运动后活性氧和自由基的增加已得到明确证实，但对抗氧化防御的影响以及对大分子的氧化损伤仍有待充分阐明。因此，本研究的目的是阐明一场超级马拉松比赛对32名跑步者氧化应激血浆标志物及其赛后恢复的影响，特别关注性别和年龄效应。为此，在比赛前、终点线以及赛后24小时和48小时测量了抗氧化酶谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）的活性，以及脂质过氧化产物丙二醛（MDA）和羰基（CG）含量。我们报告了赛后以及48小时后脂质和蛋白质（MDA和CG）的氧化损伤增加。此外，赛后GR活性增加。在整个研究过程中，未观察到跑步者血浆GPx活性的变化。最后，我们观察到了关于大分子损伤的性别和年龄差异，但在测量的抗氧化酶方面未发现差异。我们的结果表明，氧化应激的几个基础血浆标志物可能与超长耐力赛后肌肉损伤的程度有关，也可能影响肌肉力量的演变。
