Tampere Centre for Child Health Research, Tampere University and Tampere University Hospital, 33014 Tampere, Finland.
Celiac Disease Research Center, Faculty of Medicine and Health Technology, Tampere University, 33520 Tampere, Finland.
Nutrients. 2021 Feb 27;13(3):776. doi: 10.3390/nu13030776.
Anemia is a frequent finding in children with celiac disease but the detailed pathophysiological mechanisms in the intestine remain obscure. One possible explanation could be an abnormal expression of duodenal iron transport proteins. However, the results have so far been inconsistent. We investigated this issue by comparing immunohistochemical stainings of duodenal cytochrome B (DCYTB), divalent metal transporter 1 (DMT1), ferroportin, hephaestin and transferrin receptor 1 (TfR1) in duodenal biopsies between 27 children with celiac disease and duodenal atrophy, 10 celiac autoantibody-positive children with potential celiac disease and six autoantibody-negative control children. Twenty out of these 43 subjects had anemia. The expressions of the iron proteins were investigated with regard to saturation and the percentage of the stained area or stained membrane length of the enterocytes. The results showed the stained area of ferroportin to be increased and the saturation of hephaestin to be decreased in celiac disease patients compared with controls. There were no differences in the transporter protein expressions between anemic and non-anemic patients. The present results suggest an iron status-independent alteration of ferroportin and hephaestin proteins in children with histologically confirmed celiac disease.
贫血是乳糜泻患儿的常见表现，但肠道中详细的病理生理机制仍不清楚。一种可能的解释是十二指肠铁转运蛋白的异常表达。然而，到目前为止，结果并不一致。我们通过比较 27 例乳糜泻伴十二指肠萎缩患儿、10 例乳糜泻自身抗体阳性的潜在乳糜泻患儿和 6 例自身抗体阴性的对照组患儿的十二指肠活检组织中十二指肠细胞色素 B（DCYTB）、二价金属转运蛋白 1（DMT1）、亚铁转运蛋白、hephaestin 和转铁蛋白受体 1（TfR1）的免疫组织化学染色，来研究这个问题。其中 20 例患儿有贫血。用铁蛋白的饱和度和肠上皮细胞染色面积或染色膜长度的百分比来研究铁蛋白的表达。结果表明，与对照组相比，乳糜泻患者的亚铁转运蛋白染色面积增加，hephaestin 的饱和度降低。在贫血和非贫血患者之间，转运蛋白的表达没有差异。本研究结果提示，在组织学确诊的乳糜泻患儿中，存在铁状态无关的亚铁转运蛋白和 hephaestin 蛋白改变。
