The Kamila Gonczarowski Institute of Gastroenterology, Assaf Harofeh Medical Center and Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
School of Nutritional Sciences, Faculty of Agriculture, The Hebrew University of Jerusalem, Jerusalem, Israel.
J Diabetes Res. 2018 Mar 6;2018:7494821. doi: 10.1155/2018/7494821. eCollection 2018.
The role of iron transport proteins in the pathogenesis of anemia in patients with diabetes mellitus (T2DM) is still unclear. We investigated the expression of duodenal transporter proteins in diabetic patients with and without iron deficiency anemia (IDA). . Overall, 39 patients were included: 16 with T2DM and IDA (group A), 11 with T2DM without IDA (group B), and 12 controls (group C). Duodenal mucosal expression of divalent metal transporter 1 (DMT1), ferroportin 1 (FPN), hephaestin (HEPH), and transferrin receptor 1 (TfR) was evaluated by Western blotting. Chronic disease activity markers were measured as well. . FPN expression was increased in group A compared to group B and controls: 1.17 (0.72-1.46), 0.76 (0.53-1.04), and 0.71 (0.64-0.86), respectively ( = 0.011). TfR levels were over expressed in groups A and B compared to controls: 0.39 (0.26-0.61), 0.36 (0.24-0.43), and 0.18 (0.16-0.24), respectively, ( = 0.004). The three groups did not differ significantly with regard to cellular HEPH and DMT1 expression. The normal CRP and serum ferritin levels, accompanied with normal FPN among diabetic patients without IDA, do not support the association of IDA with chronic inflammatory state. . In patients with T2DM and IDA, duodenal iron transport protein expression might be dependent on body iron stores rather than by chronic inflammation or diabetes per se.
铁转运蛋白在糖尿病患者（T2DM）贫血发病机制中的作用尚不清楚。我们研究了伴有和不伴有缺铁性贫血（IDA）的糖尿病患者十二指肠转运蛋白的表达。共纳入 39 例患者：16 例 T2DM 伴 IDA（A 组）、11 例 T2DM 不伴 IDA（B 组）和 12 例对照（C 组）。采用 Western blot 法评估十二指肠黏膜二价金属转运蛋白 1（DMT1）、亚铁转运蛋白 1（FPN）、hephaestin（HEPH）和转铁蛋白受体 1（TfR）的表达。还测定了慢性疾病活动标志物。与 B 组和对照组相比，A 组 FPN 表达增加：分别为 1.17（0.72-1.46）、0.76（0.53-1.04）和 0.71（0.64-0.86），差异有统计学意义（ = 0.011）。与对照组相比，A 组和 B 组 TfR 水平升高：分别为 0.39（0.26-0.61）、0.36（0.24-0.43）和 0.18（0.16-0.24），差异有统计学意义（ = 0.004）。三组之间细胞 HEPH 和 DMT1 表达无显著差异。糖尿病患者无 IDA 时，正常 CRP 和血清铁蛋白水平及正常 FPN 不支持 IDA 与慢性炎症状态有关。在 T2DM 伴 IDA 患者中，十二指肠铁转运蛋白表达可能依赖于机体铁储存，而不是慢性炎症或糖尿病本身。
