Yong V W, Sekiguchi S, Kim M W, Kim S U
Department of Medicine, University of British Columbia, Vancouver, Canada.
J Neurosci Res. 1988 Feb;19(2):187-94. doi: 10.1002/jnr.490190203.
The effects of phorbol esters have been attributed to the activation of the enzyme protein kinase C. While much has been described for the actions of phorbol esters on neurons and synaptic transmission, sparse data exist on the effects of phorbol esters on oligodendrocytes, the cells that make and maintain myelin in the central nervous system. In this report, we show that 10 and 100 nM of a phorbol ester, 4 beta-phorbol-12,13-dibutyrate, extensively enhanced process formation by cultured bovine oligodendrocytes. This effect was blocked by two inhibitors of protein kinase C, sodium heparin and polymixin B. We propose the hypothesis that activation of protein kinase C is an important process that leads to the differentiation of oligodendrocytes and the formation of myelin in vivo.
佛波酯的作用归因于酶蛋白激酶C的激活。虽然关于佛波酯对神经元和突触传递的作用已有很多描述，但关于佛波酯对少突胶质细胞（中枢神经系统中形成和维持髓鞘的细胞）作用的数据却很少。在本报告中，我们表明10 nM和100 nM的佛波酯4β-佛波醇-12,13-二丁酸酯可广泛增强培养的牛少突胶质细胞的突起形成。这种作用被蛋白激酶C的两种抑制剂肝素钠和多粘菌素B所阻断。我们提出这样的假说，即蛋白激酶C的激活是一个重要过程，它导致少突胶质细胞在体内分化并形成髓鞘。
