Laboratory of Molecular and Preventive Dentistry, Department of Preventive Dentistry, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, 639 Zhizaoju Road, Huangpu District, Shanghai 200011, China.
Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
Dent Mater. 2021 May;37(5):849-862. doi: 10.1016/j.dental.2021.02.009. Epub 2021 Mar 3.
To evaluate preventive effects of S-PRG-filled resin-composites on surrounding tooth demineralization and interfacial bacterial penetration.
Cavities were prepared on enamel or dentin blocks and treated by BeautiBond Universal (Shofu) and filled by resin-composites containing S-PRG filler (Shofu) at 0 wt% (P0, control), 10 wt% (P10), 30 wt% (P30), 50 wt% (P50) or 70 wt% (P70). The specimens were demineralized by Streptococcus mutans biofilms for 12 days. Wall lesion depth (WL-depth) and outer lesion depth (OL-depth) were measured from fluorescence microscopy. Interfacial bacterial penetration area (BPA) was evaluated from confocal laser scanning microscopy. Wall lesion hardness (WL-hardness) and outer lesion hardness (OL-hardness) were measured with a Vickers microhardness tester. Elemental analysis of resins and surrounding lesions was conducted by scanning electron microscopy and energy dispersive X-ray spectrometry.
Resin-composites in P70 contained significantly more F, Na and Al than that in P0 and P10. P70, P50 and P30 showed significantly lower WL-depth than P0. P70 showed significantly lower OL-depth, fewer Ca and P loss in lesions than P0. P70 showed significantly higher WL-hardness and OL-hardness in dentin specimens than P0. Enamel margins had a higher gap frequency than dentin margins. However, BPA did not show significant differences among the groups.
Resin-composites containing S-PRG fillers at ≥30 wt% can inhibit wall lesions and that at 70 wt% can further decrease outer lesions, reduce Ca and P loss in lesions and increase surrounding dentin hardness. However, S-PRG-filled resin-composites does not inhibit interfacial biofilm penetration after a short-term demineralization period.
评价 S-PRG 填充型树脂复合材料对邻面牙体脱矿及界面细菌渗透的预防作用。
在釉质或牙本质块上制备窝洞，用 BeautiBond Universal（Shofu）处理，并用 0 wt%（P0，对照组）、10 wt%（P10）、30 wt%（P30）、50 wt%（P50）或 70 wt%（P70）S-PRG 填充型树脂复合材料填充。将标本用变异链球菌生物膜脱矿 12 天。从荧光显微镜测量壁面损伤深度（WL-depth）和外损伤深度（OL-depth）。从共聚焦激光扫描显微镜评估界面细菌渗透面积（BPA）。用维氏硬度计测量壁面损伤硬度（WL-hardness）和外损伤硬度（OL-hardness）。用扫描电子显微镜和能量色散 X 射线光谱仪对树脂和周围病变进行元素分析。
P70 组树脂复合材料中 F、Na 和 Al 的含量明显高于 P0 和 P10 组。P70 组的 WL-depth 明显低于 P0 组。P70 组的 OL-depth、病变中 Ca 和 P 的损失明显少于 P0 组。P70 组牙本质标本的 WL-hardness 和 OL-hardness 明显高于 P0 组。与牙本质边缘相比，釉质边缘的间隙频率更高。然而，各组间的 BPA 无显著差异。
含 S-PRG 填料的树脂复合材料含量≥30 wt%时可抑制壁面病变，含量 70 wt%时可进一步减少外病变，减少病变中 Ca 和 P 的损失，增加周围牙本质硬度。然而，在短期脱矿期后，S-PRG 填充型树脂复合材料并不能抑制界面生物膜渗透。
