Pushp Pallavi, Bhaskar Rakesh, Kelkar Samruddhi, Sharma Neelesh, Pathak Devendra, Gupta Mukesh Kumar
Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Odisha, India.
Department of Biotechnology, Institute of Engineering and Technology, Bundelkhand University, Jhansi, Uttar Pradesh, India.
Biotechnol Bioeng. 2021 Jun;118(6):2312-2325. doi: 10.1002/bit.27743. Epub 2021 Mar 25.
Polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) are the two most investigated biopolymers for various tissue engineering applications. However, their poor tensile strength renders them unsuitable for cardiac tissue engineering (CTE). In this study, we developed and evaluated PVA-PVP-based patches, plasticized with glycerol or propylene glycol (0.1%-0.4%; v:v), for their application in CTE. The cardiac patches were evaluated for their physico-chemical (weight, thickness, folding endurance, FT-IR, and swelling behavior) and mechanical properties. The optimized patches were characterized for their ability to support in vitro attachment, viability, proliferation, and beating behavior of neonatal mouse cardiomyocytes (CMs). In vivo evaluation of the cardiac patches was done under the subcutaneous skin pouch and heart of rat models. Results showed that the optimized molar ratio of PVA:PVP with plasticizers (0.3%; v-v) resulted in cardiac patches, which were dry at room temperature and had desirable folding endurance of at least 300, a tensile strength of 6-23 MPa and, percentage elongation at break of more than 250%. Upon contact with phosphate-buffered saline, these PVA-PVP patches formed hydrogel patches having the tensile strength of 1.3-3.0 MPa. The patches supported the attachment, viability, and proliferation of primary neonatal mouse CMs and were nonirritant and noncorrosive to cardiac cells. In vivo transplantation of cardiac patches into a subcutaneous pouch and on the heart of rat models revealed them to be biodegradable, biocompatible, and safe for use in CTE applications.
聚乙烯醇（PVA）和聚乙烯吡咯烷酮（PVP）是用于各种组织工程应用研究最多的两种生物聚合物。然而，它们较差的拉伸强度使其不适用于心脏组织工程（CTE）。在本研究中，我们开发并评估了用甘油或丙二醇（0.1%-0.4%；体积比）增塑的基于PVA-PVP的贴片在CTE中的应用。对心脏贴片的物理化学性质（重量、厚度、耐折叠性、傅里叶变换红外光谱和溶胀行为）和力学性能进行了评估。对优化后的贴片支持新生小鼠心肌细胞（CMs）体外附着、活力、增殖和跳动行为的能力进行了表征。在大鼠模型的皮下皮肤袋和心脏下对心脏贴片进行了体内评估。结果表明，PVA:PVP与增塑剂（0.3%；体积比）的优化摩尔比产生的心脏贴片在室温下干燥，具有至少300次的理想耐折叠性、6-23 MPa的拉伸强度和超过250%的断裂伸长率。与磷酸盐缓冲盐水接触后，这些PVA-PVP贴片形成了拉伸强度为1.3-3.0 MPa的水凝胶贴片。这些贴片支持原代新生小鼠CMs的附着、活力和增殖，并且对心脏细胞无刺激性和腐蚀性。将心脏贴片体内移植到大鼠模型的皮下袋和心脏上表明它们可生物降解、生物相容且在CTE应用中使用安全。
