Eugene & Marilyn Glick Eye Institute, Department of Ophthalmology, Indiana University School of Medicine, USA.
Eugene & Marilyn Glick Eye Institute, Department of Ophthalmology, Indiana University School of Medicine, USA; Department of Pharmacology and Toxicology, Indiana University School of Medicine, USA.
Exp Eye Res. 2021 May;206:108530. doi: 10.1016/j.exer.2021.108530. Epub 2021 Mar 3.
Noninvasive in vivo imaging of the mouse retina is essential for eye research. However, imaging the mouse fundus is challenging due to its small size and requires specialized equipment, maintenance, and training. These issues hinder the routine evaluation of the mouse retina. In this study, we developed a noncontact imaging system consisting of a smartphone, a 90D condensing lens, a homemade light diaphragm, a tripod, and a Bluetooth remote. With minimal training, examiners were able to capture fundus images from the mouse retina. We also found that fundus images captured using our system from wild type mice, mice with laser-induced retinal injury, and a mouse model of retinitis pigmentosa showed a quality similar to those captured using a commercial fundus camera. These images enabled us to identify normal structures and pathological changes in the mouse retina. Additionally, fluorescein angiography was possible with the smartphone system. We believe that the smartphone imaging system is low cost, simple, accessible, easy to operate, and suitable for the routine screening and examination of the mouse eye.
非侵入式活体小鼠视网膜成像对于眼部研究至关重要。然而，由于小鼠眼底较小，需要专门的设备、维护和培训，因此对其进行成像具有挑战性。这些问题阻碍了对小鼠视网膜的常规评估。在这项研究中，我们开发了一种由智能手机、90D 聚光透镜、自制光阑、三脚架和蓝牙遥控器组成的非接触式成像系统。经过少量培训，检查人员就能从小鼠视网膜上获取眼底图像。我们还发现，使用我们的系统从野生型小鼠、激光诱导视网膜损伤的小鼠和色素性视网膜炎小鼠模型中获取的眼底图像质量与使用商用眼底相机获取的图像质量相似。这些图像使我们能够识别小鼠视网膜的正常结构和病理变化。此外，智能手机系统还可以进行荧光素血管造影。我们相信，智能手机成像系统具有成本低、操作简单、易于使用、适用于常规筛查和检查小鼠眼部的优点。
