College of Medicine, Henan Engineering Research Center of Funiu Mountain's Medicinal Resources Utilization and Molecular Medicine, Pingdingshan University, Pingdingshan, Henan 467000, China.
College of Life Science, Xinyang Normal University, Xinyang, Henan 464000, China.
Int J Biol Macromol. 2021 May 15;179:259-269. doi: 10.1016/j.ijbiomac.2021.02.217. Epub 2021 Mar 3.
Anoectochilus roxburghii is a traditional herb in China that can be potentially used to treat diabetes. A novel polysaccharide ARLP-W was isolated from Anoectochilus roxburghii by chromatography on DEAE-52 cellulose. Chemical analysis indicated that ARLP-W (8.1 × 10 Da) was mainly composed of mannose and glucose. The main linkages of glycosidic bonds of ARLP-W were β-1, 4-Manp and α-1, 4-Glcp. The terminal Glcp was connected to Manp-via O-3. RT-qPCR and western blotting analysis showed that ARLP-W caused a significant reduction in the levels of the key gluconeogenesis enzymes phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase) in the liver. The results of the insulin resistance tests indicated that ARLP-W increased glucose absorption. These results indicate that ARLP-W has a good therapeutic effect on type 2 diabetes and can assist with further development and application treatment of diabetes.
铁皮石斛是中国的一种传统草药，具有治疗糖尿病的潜力。一种新型多糖 ARLP-W 从铁皮石斛中通过 DEAE-52 纤维素层析分离得到。化学分析表明，ARLP-W（8.1×10 Da）主要由甘露糖和葡萄糖组成。ARLP-W 的糖苷键的主要连接方式为β-1,4-Manp 和α-1,4-Glcp。末端 Glcp 通过 O-3 连接到 Manp。实时 qPCR 和 Western blotting 分析表明，ARLP-W 可显著降低肝脏中关键糖异生酶磷酸烯醇丙酮酸羧激酶（PEPCK）和葡萄糖 6-磷酸酶（G6Pase）的水平。胰岛素抵抗试验结果表明，ARLP-W 增加了葡萄糖的吸收。这些结果表明，ARLP-W 对 2 型糖尿病有较好的治疗效果，可辅助进一步开发和应用于糖尿病的治疗。
