State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
Institute of pathology, Lanzhou university, Lanzhou 730000, PR China.
J Ethnopharmacol. 2017 Apr 18;202:192-199. doi: 10.1016/j.jep.2017.03.012. Epub 2017 Mar 10.
Anoectochilus roxburghii has been used as a health food and a herb for treatment diabetes in China for hundreds years. Anoectochilus roxburghii polysaccharose (ARP) is the major active component of the plant.
The present study investigated the vascular protection of ARP in vivo and in vitro experiments.
Hypoglycemic activity of ARP was examined in diabetic mice. Moreover, the further vascular protective effects in vitro were investigated in human umbilical vein endothelial cells (HUVECs) stimulated by high glucose (HG, 35mM).
Compared with untreated diabetic mice, ARP (100 or 300mg/kg) caused a significant decrease in blood glucose levels. Histological examination showed that ARP ameliorated endothelial damage to some extent, especially ARP at dosage of 300mg/kg. In vitro assay, pretreatment with ARP (10, 20 and 30μg/mL) markedly inhibited generations of reactive oxygen species (ROS), monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1) in HG-induced HUVECs. ARP pretreatment not only suppressed HG-induced matrix metalloproteinases (MMPs) activity via increasing the expression of the tissue inhibitors of MMPs (TIMPs), but also adjusted the MMPs/TIMPs balance to maintain homeostasis of vascular structure. Moreover, pretreatment with ARP could significantly reduce p-NF-κB p65, p-p38 MAPK expression levels in HG-induced HUVECs.
The vascular protective effects of ARP might be associated with NF-κB and p38 MAPK pathway. ARP might be used as useful substance in the treatment of vasculopathy in diabetic patients.
铁皮石斛作为一种保健品和治疗糖尿病的草药在中国已使用了数百年。铁皮石斛多糖（ARP）是该植物的主要活性成分。
本研究通过体内和体外实验研究了 ARP 的血管保护作用。
在糖尿病小鼠中检测 ARP 的降血糖活性。此外，还在高糖（HG，35mM）刺激的人脐静脉内皮细胞（HUVEC）中进一步研究了其体外的血管保护作用。
与未治疗的糖尿病小鼠相比，ARP（100 或 300mg/kg）可显著降低血糖水平。组织学检查表明，ARP 在一定程度上改善了内皮损伤，尤其是 ARP 剂量为 300mg/kg 时。体外试验表明，ARP（10、20 和 30μg/mL）预处理可显著抑制 HG 诱导的 HUVEC 中活性氧（ROS）、单核细胞趋化蛋白-1（MCP-1）和细胞间黏附分子-1（ICAM-1）的产生。ARP 预处理不仅通过增加基质金属蛋白酶抑制剂（TIMP）的表达来抑制 HG 诱导的基质金属蛋白酶（MMPs）活性，而且还调节了 MMPs/TIMP 平衡，以维持血管结构的内稳态。此外，ARP 预处理可显著降低 HG 诱导的 HUVEC 中 p-NF-κB p65、p-p38MAPK 的表达水平。
ARP 的血管保护作用可能与 NF-κB 和 p38MAPK 通路有关。ARP 可能可作为治疗糖尿病患者血管病变的有用物质。
