Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Rodovia Araraquara-Jaú km 1, Araraquara 14801-902, SP, Brazil.
Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Rodovia Araraquara-Jaú km 1, Araraquara 14801-902, SP, Brazil; Instituto de Biociências, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Rua Dr. Antônio Celso Wagner Zanin, 250, Distrito de Rubião Júnior, Botucatu 18618-689, SP, Brazil.
Infect Genet Evol. 2021 Jul;91:104798. doi: 10.1016/j.meegid.2021.104798. Epub 2021 Mar 4.
There are currently 156 species, grouped into 18 genera and five tribes included in the subfamily Triatominae. All of them are potential vectors of Chagas disease. Triatoma is paraphyletic and the species of this genus have been grouped into complexes and subcomplexes. Triatoma brasiliensis complex is a monophyletic group composed of eight taxa: T. b. brasiliensis, T. b. macromelanosoma, T. juazeirensis, T. sherlocki, T. petrocchiae, T. lenti, T. bahiensis, T. melanica. Experimental crosses have helped in systematic, taxonomic and evolutionary issues of these vectors. Based on this, we carried out experimental crosses between T. lenti and four other species of the T. brasiliensis complex and analyzed the segregation pattern of phenotypic characteristics of T. lenti, T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica in the hybrids. The hybrids resulting from the crosses between T. b. brasiliensis ♀ x T. lenti ♂, T. juazeirensis ♀ x T. lenti ♂, and T. melanica ♀ x T. lenti ♂ showed segregation of characteristics of both parental species. On the other hand, the hybrids between T. lenti ♀ x T. juazeirensis ♂, T. b. macromelasoma ♀ x T. lenti ♂, and T. lenti ♀ x T. melanica ♂ showed a specific pattern of T. lenti, T. lenti and T. melanica, respectively. Thus, the pattern of segregation of morphological characteristics between species of the T. brasiliensis complex was characterized. These results highlight the importance of integrative taxonomy for the correct identification of Chagas disease vectors grouped in the T. brasiliensis complex if natural hybridization events occur.
目前，在三锥虫亚科中共有 156 个种，分为 18 属 5 个族，均为传播恰加斯病的潜在媒介。三锥虫是并系的，该属的物种已被分为复合物和亚复合物。巴西三锥虫复合体是一个单系群，由 8 个分类群组成：T. b. brasiliensis、T. b. macromelanosoma、T. juazeirensis、T. sherlocki、T. petrocchiae、T. lenti、T. bahiensis、T. melanica。实验性杂交有助于解决这些媒介的系统学、分类学和进化问题。基于此，我们在 T. lenti 与巴西三锥虫复合体的其他四个种之间进行了实验性杂交，并分析了 T. lenti、T. b. brasiliensis、T. b. macromelasoma、T. juazeirensis 和 T. melanica 杂种在表型特征分离模式中的表现。T. b. brasiliensis♀×T. lenti♂、T. juazeirensis♀×T. lenti♂和 T. melanica♀×T. lenti♂杂交产生的杂种显示出双亲种特征的分离。另一方面，T. lenti♀×T. juazeirensis♂、T. b. macromelasoma♀×T. lenti♂和 T. lenti♀×T. melanica♂杂交产生的杂种分别显示出 T. lenti、T. lenti 和 T. melanica 的特定模式。因此，巴西三锥虫复合体种间形态特征分离模式的特征得到了描述。这些结果强调了如果发生自然杂交事件，综合分类学对于正确识别恰加斯病传播媒介在巴西三锥虫复合体中的重要性。
