Bjerre-Bastos J J, Nielsen H B, Andersen J R, Karsdal M, Bay-Jensen A-C, Boesen M, Mackey A L, Byrjalsen I, Bihlet A R
Xlab, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Nordic Bioscience Clinical Development, Herlev, Denmark.
Department of Anesthesia, Zealand University Hospital Roskilde, Denmark; Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Denmark.
Osteoarthritis Cartilage. 2021 Jul;29(7):986-994. doi: 10.1016/j.joca.2021.02.569. Epub 2021 Mar 3.
To investigate acute changes in biochemical markers of cartilage turnover in response to moderate intensity exercise with and without joint impact in humans with knee osteoarthritis.
We conducted a randomized, cross-over, exploratory clinical study. Twenty subjects with knee osteoarthritis (OA) were randomized, of which twenty completed 30 min of cycling and 15 completed 30 min of running on days 1 week apart. Fasting blood samples were taken before, immediately after and 1, 2, 3, and 24 h after activity was initiated. Midstream spot urine was sampled before and after activity. Serum samples were analyzed for concentrations of fragment of type II collagen degradation, C2M, fragment of type VI collagen degradation, C6M, cartilage oligomeric matrix protein, COMP, marker of type II collagen formation, PRO-C2, and urine for marker of crosslinked type II collagen degradation, CTX-II. To establish a reference, all subjects had similar samples taken during rest on a separate day. Data was analyzed in a restricted maximum likelihood based random effects linear mixed model.
C2M trended to increase after cycling compared running (13.49%, 95%CI: -0.36-27.34%) and resting (12.88%, 95%CI: 0.2-25.6%) and the type II collagen formation/degradation ratio switched towards degradation after cycling, but not running. C6M trended to decrease after cycling (-8.1%, 95%CI: -14.8 to -1.4%) and running (-6.8%, 95%CI: -14.16-0.55%).
In persons with knee OA moderate intensity exercise without joint impact may induce acute changes in circulating levels of biochemical markers reflecting type II and VI collagen degradation.
研究膝关节骨关节炎患者在进行有或无关节冲击的中等强度运动后，软骨代谢生化标志物的急性变化。
我们进行了一项随机、交叉、探索性临床研究。20名膝关节骨关节炎（OA）患者被随机分组，其中20人在相隔1周的日子里完成了30分钟的骑行，15人完成了30分钟的跑步。在活动开始前、活动结束后即刻以及活动开始后1、2、3和24小时采集空腹血样。在活动前后采集中段晨尿。分析血清样本中II型胶原降解片段C2M、VI型胶原降解片段C6M、软骨寡聚基质蛋白COMP、II型胶原形成标志物PRO-C2的浓度，并分析尿液中交联II型胶原降解标志物CTX-II的浓度。为建立对照，所有受试者在单独一天的休息期间采集了类似样本。数据采用基于限制最大似然法的随机效应线性混合模型进行分析。
与跑步（13.49%，95%CI：-0.36 - 27.34%）和休息（12.88%，95%CI：0.2 - 25.6%）相比，骑行后C2M有升高趋势，且骑行后II型胶原形成/降解比转向降解，而跑步后未出现这种情况。骑行（-8.1%，95%CI：-14.8至-1.4%）和跑步（-6.8%，95%CI：-14.16 - 0.55%）后C6M有下降趋势。
对于膝关节OA患者，无关节冲击的中等强度运动可能会引起反映II型和VI型胶原降解的循环生化标志物水平的急性变化。
