Caspase-3 相关凋亡可预防活体肝供体大鼠模型中的病理性再生。
Caspase-3-related apoptosis prevents pathological regeneration in a living liver donor rat model.
机构信息
Centro de Investigaciones Biomédicas, Universidad Veracruzana, Xalapa Veracruz, Mexico.
Department of Biosciences, Rajiv Gandhi University of Knowledge Technologies (RGUKT), Srikakulam, India.
出版信息
Adv Med Sci. 2021 Mar;66(1):176-184. doi: 10.1016/j.advms.2021.02.003. Epub 2021 Mar 3.
PURPOSE
The main goal of this study was to determine the relationship of cleaved-caspase-3 (C3)-related apoptosis and hepatic proliferation, during the liver repopulation in a living liver donor rat model.
MATERIAL/METHODS: Thirty-three animals were randomized into eleven groups and evaluated on postoperative from 3 h until 384 h after 30%-partial hepatectomy (30%-PHx). Liver sections (5 μm) were processed by hematoxylin-eosin, and immunostaining for C3, accompanied by hepatic function test. C3 content and the hepatic lobule enlargement were analyzed by optical density, followed by cell counting.
RESULTS
Transient variations of alanine transferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were found. Significant increase in the C3 levels, and cell nuclei number, were detected at 12 h and 48 h after 30%-PHx, evidencing a correlation of p = -0.3679.
CONCLUSION
In the 30%-PHx rat model, C3-related apoptosis prevents proliferative pathological conditions during the hepatic lobule re-modeling.
目的
本研究的主要目的是确定在活体肝供体大鼠模型中，肝部分切除（30% - PHx）后，C3 相关凋亡与肝增殖之间的关系。
材料/方法：33 只动物随机分为 11 组，在术后 3 h 至 30% - PHx 后 384 h 进行评估。通过苏木精-伊红、C3 免疫染色以及肝功能试验对肝组织切片（5 μm）进行处理。通过光密度分析 C3 含量和肝小叶增大，并进行细胞计数。
结果
丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和乳酸脱氢酶（LDH）的变化是短暂的。在 30% - PHx 后 12 h 和 48 h 时，C3 水平和细胞核数量显著增加，相关系数 p = -0.3679。
结论
在 30% - PHx 大鼠模型中，C3 相关凋亡可防止肝小叶重塑过程中的增殖性病理状态。
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