Mezera Vojtěch, Kučera Otto, Moravcová Alena, Peterová Eva, Červinková Zuzana
Department of Physiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Simkova 870, 500 38, Hradec Kralove 1, Czech Republic,
Dig Dis Sci. 2014 May;59(5):976-85. doi: 10.1007/s10620-013-2966-5. Epub 2013 Dec 8.
Two-thirds partial hepatectomy (PHx) is an established model for the study of liver regeneration after resection. This process is accompanied by oxidative stress.
In our study, we tested the effect of epigallocatechin gallate (EGCG), a green tea antioxidant, on the early phase of liver regeneration after PHx.
Male Wistar rats were divided into five groups: (I) laparotomy + water for intraperitoneal injections, (II) laparotomy + EGCG 50 mg/kg body weight, (III) PHx + water for injections, (IV) PHx + EGCG 20 mg/kg and (V) PHx + EGCG 50 mg/kg, for 3 consecutive days. The rats were killed 24 h after surgery. Biochemical analysis of rat sera was performed. Histological samples were stained with hematoxylin & eosin and bromodeoxyuridine (BrdU). In hepatectomized rats, we also measured plasma malondialdehyde, tissue malondialdehyde, glutathione and cytokines levels, the activity of caspases 3/7, expression of Nqo-1 and HO-1 genes at the mRNA level, and expression of p21, p-p27 and p-p53 genes at the protein level.
We observed lower accumulation of BrdU in group V when compared to groups III and IV. The activity of caspases 3/7 and expression of p-p53 were lower in group V than in groups III and IV. Tissue levels of IL-6 were lower in group V when compared to group III. Significant differences were not noted in other parameters.
Administration of EGCG did not stimulate early phase liver regeneration in rats after PHx. There was even lower DNA synthesis in the group treated with a high dose of EGCG.
三分之二肝部分切除术（PHx）是研究肝脏切除术后肝再生的既定模型。这一过程伴随着氧化应激。
在我们的研究中，我们测试了绿茶抗氧化剂表没食子儿茶素没食子酸酯（EGCG）对PHx后肝再生早期阶段的影响。
将雄性Wistar大鼠分为五组：（I）剖腹术 + 腹腔注射用水，（II）剖腹术 + 50 mg/kg体重的EGCG，（III）PHx + 注射用水，（IV）PHx + 20 mg/kg的EGCG，以及（V）PHx + 50 mg/kg的EGCG，连续3天。术后24小时处死大鼠。对大鼠血清进行生化分析。组织学样本用苏木精和伊红以及溴脱氧尿苷（BrdU）染色。在肝切除大鼠中，我们还测量了血浆丙二醛、组织丙二醛、谷胱甘肽和细胞因子水平、半胱天冬酶3/7的活性、Nqo-1和HO-1基因在mRNA水平的表达，以及p21、p-p27和p-p53基因在蛋白质水平的表达。
与III组和IV组相比，我们观察到V组中BrdU的积累较低。V组中半胱天冬酶3/7的活性和p-p53的表达低于III组和IV组。与III组相比，V组中IL-6的组织水平较低。在其他参数中未观察到显著差异。
PHx后给予EGCG并未刺激大鼠肝再生的早期阶段。在高剂量EGCG处理的组中甚至有更低的DNA合成。
