University of São Paulo, São Carlos Institute of Physics, Brazil.
University of São Paulo, São Carlos Institute of Physics, Brazil.
J Photochem Photobiol B. 2021 Apr;217:112170. doi: 10.1016/j.jphotobiol.2021.112170. Epub 2021 Feb 27.
A combination of metabolic modifications by light stimulus and photodynamic action is very attractive. Photobiomodulation therapy (PBMT) comprehends a vast range of applications and has been shown to be suitable to ease morbidities caused by chemotherapy and radiation, such as mucositis and dermatitis. The current study investigates the effects of near-infrared PBMT combined with porphyrin-based photodynamic therapy (PDT) in squamous cell carcinoma cell lines SCC-25 and SCC-4. The aim is to evaluate the potential of this combination to improve PDT outcome by increasing cell toxicity. Many techniques were used to verify the combined effect. Photobiomodulation (PBM) enhanced PDT action in SCC-25 cells by increasing photosensitizer (PS) uptake and production of reactive oxygen species (ROS). The equivalent was not seen in SCC-4 cells compared to the PDT only group. We believe these effects are strongly related to the interval of application between PBMT, PS incubation and PDT. Additionally, the effect of ascorbic acid on preventing PBM effects in PDT shows that ROS play an important role in the early mechanisms of PBM-PDT. Therefore, we believe PBM-PDT combination is worth exploring, for its benefit-cost ratio and simple protocols, along with the possibility of improvement in treatment resuts.
光刺激和光动力作用的代谢修饰联合非常有吸引力。光生物调节疗法（PBMT）包含广泛的应用，已被证明可缓解化疗和放疗引起的多种疾病，如黏膜炎和皮炎。本研究探讨了近红外 PBMT 联合基于卟啉的光动力疗法（PDT）在鳞状细胞癌细胞系 SCC-25 和 SCC-4 中的作用。目的是通过增加细胞毒性来评估这种联合疗法提高 PDT 效果的潜力。为了验证联合效应，我们使用了许多技术。光生物调节（PBM）通过增加光敏剂（PS）摄取和活性氧（ROS）的产生来增强 SCC-25 细胞中的 PDT 作用。与单独 PDT 组相比，在 SCC-4 细胞中没有观察到这种作用。我们认为这些效应与 PBMT、PS 孵育和 PDT 之间的应用间隔密切相关。此外，抗坏血酸对预防 PDT 中 PBM 效应的作用表明，ROS 在 PBM-PDT 的早期机制中起重要作用。因此，我们认为 PBMT-PDT 联合治疗具有很高的研究价值，因为其具有成本效益比和简单的方案，同时还有可能改善治疗效果。
