Department of Otorhinolaryngology, Medical University of Innsbruck, 6020 Innsbruck, Austria.
Int J Mol Sci. 2018 Apr 7;19(4):1107. doi: 10.3390/ijms19041107.
Photodynamic therapy (PDT) is suggested to have an impact on the treatment of early stage head and neck cancers (HNSCC). We investigated the effect of PDT with methylene blue (MB) and a diode laser (660 nm) as the laser source on HNSCC cell lines as an in vitro model of surface oral squamous cell carcinoma. Cell-cultures were exposed to 160 µM MB for 4 min and to laser light for 8 min. Viability was proven via cell viability assay and clonogenic survival via clone counting assay. The combination of MB and diode laser evidenced high efficient loss of cell viability by 5% of the control, while treatment with the same concentration of MB for 4 min alone showed a viability of 46% of the control. In both SCC-25 and Detroit 562 HNSCC cells, MB combined with the laser allowed a significant abrogation of clonogenic growth ( < 0.01), especially in the case of Detroit 562 cells less than 1% of the suspension plated cells were able to grow tumor cell nests. Multiresistant (Detroit 562) HNSCC cells expressing cancer stem cell markers are sensitive to MB/red laser combined PDT.
光动力疗法（PDT）被认为对早期头颈部癌症（HNSCC）的治疗有影响。我们研究了亚甲蓝（MB）和二极管激光（660nm）作为激光源的 PDT 对 HNSCC 细胞系的影响，作为口腔表面鳞状细胞癌的体外模型。细胞培养物用 160µM 的 MB 孵育 4 分钟，并用激光照射 8 分钟。通过细胞活力测定法证明活力，通过克隆计数测定法证明集落存活。MB 和二极管激光的组合通过 5%的对照证明了高效的细胞活力丧失，而单独用相同浓度的 MB 处理 4 分钟显示出对照的 46%的活力。在 SCC-25 和 Detroit 562 HNSCC 细胞中，MB 联合激光能够显著阻断集落生长（<0.01），特别是在 Detroit 562 细胞的情况下，少于 1%的悬浮细胞能够生长肿瘤细胞巢。表达癌症干细胞标志物的多药耐药（Detroit 562）HNSCC 细胞对 MB/红光激光联合 PDT 敏感。
