Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, E-30100, Murcia, Spain.
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, E-30100, Murcia, Spain.
Talanta. 2021 May 1;226:122144. doi: 10.1016/j.talanta.2021.122144. Epub 2021 Jan 28.
Paprika is considered a high-quality product being one of the most consumed spices in the world. Contamination with mycotoxins may appear due to inappropriate practices during processing or resulting from invading mould in the final manufactured products. A sample treatment based on dispersive magnetic solid-phase extraction (DMSPE) has been proposed for emerging mycotoxin determination, enniatins (ENNs) and beauvericins (BEAs), in paprika. Different magnetic nanoparticles were tested, and cellulose-ferrite nanocomposite was selected for the extraction and preconcentration of the mycotoxins. Nanocomposite was characterised using field emission scanning electron microscopy and energy dispersive X-ray spectroscopy in terms of morphology and elemental composition. High-resolution mass spectrometry allowed the quantification of the five main emerging mycotoxins and the monitoring of unexpected members of this class of toxic fungal secondary metabolites. The method has been validated, obtaining limits of quantification between 9.5 and 9.9 μg kg and testing its trueness through recovery studies, with satisfactory values of between 89.5 and 97.7%. Relative standard deviations were calculated to evaluate the intra- and inter-day precision and values lower than 8% were obtained in all cases. The analysis of 26 samples, including conventional and organic, demonstrated the presence of ENNB1 at 12.0 ± 0.6 μg kg in one of the samples studied. Other analogues ENNs and BEAs were not detected.
红辣椒被认为是一种高质量的产品，是世界上消费最多的香料之一。由于加工过程中的不当操作或最终制成品中侵入的霉菌，可能会出现霉菌毒素污染。已经提出了一种基于分散固相萃取（DMSPE）的样品处理方法，用于测定辣椒粉中的新兴霉菌毒素，包括恩镰孢菌素（ENNs）和 beauvericin（BEAs）。测试了不同的磁性纳米粒子，并选择纤维素-铁纳米复合材料用于提取和浓缩霉菌毒素。纳米复合材料的形态和元素组成用场发射扫描电子显微镜和能量色散 X 射线光谱进行了表征。高分辨率质谱法允许对五种主要的新兴霉菌毒素进行定量，并监测此类有毒真菌次生代谢物的意外成员。该方法已经过验证，获得了 9.5 至 9.9μg/kg 的定量限，并通过回收研究测试了其准确性，回收率在 89.5%至 97.7%之间。相对标准偏差用于评估日内和日间精密度，所有情况下得到的数值均低于 8%。对 26 个样品（包括常规和有机样品）的分析表明，在研究的一个样品中存在 12.0±0.6μg/kg 的 ENNB1。未检测到其他类似物 ENNs 和 BEAs。
