School of Pharmacy, Fudan University, Shanghai 201203, China.
Open Studio for Druggability Research of Marine Natural Products, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, Beijing 100049, China; School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China.
J Pharm Biomed Anal. 2021 May 10;198:113993. doi: 10.1016/j.jpba.2021.113993. Epub 2021 Feb 26.
Three maytansinoids with strong cytotoxicities, dehydrotrewiasine, maytanbutine, and trewiasine, were isolated and identified from Trewia nudiflora, and maytanbutine was obtained from this plant for the first time. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction combined with high-performance liquid chromatography (HPLC) was established to determine the three maytansinoids in T. nudiflora. The effects of major factors on the extraction efficiency of the QuEChERS method were evaluated and the optimal conditions using acetonitrile-ethyl acetate (1:1, v/v) as the extraction solvent and PestiCarb as the clean-up sorbents were established. Compared with Soxhlet extraction (SE) and ultrasonic-assisted extraction (UAE), the QuEChERS method was easy-to-operate and afforded a cleaner extract. A phenomenex HyperClone BDS C column was used for HPLC analysis. Methanol-acetonitrile-water was chosen as mobile phase for gradient elution. Method validation showed that all analytes showed good linearity (r > 0.999) over the investigated ranges and satisfactory recoveries ranging from 95.0% to 105.0%. The developed QuEChERS-HPLC method was simple, efficient, and applicable to the determination of maytansinoids in T. nudiflora.
从垂序商陆中分离鉴定出三种具有强细胞毒性的美登素类似物，即去氢美登素、美登普汀和垂序商陆素，并首次从该植物中获得美登普汀。建立了一种快速、简便、廉价、有效、耐用和安全（QuEChERS）提取与高效液相色谱（HPLC）相结合的方法，用于测定垂序商陆中的三种美登素类似物。评估了主要因素对 QuEChERS 方法提取效率的影响，并确定了最佳条件，即采用乙腈-乙酸乙酯（1:1，v/v）作为提取溶剂，并用 PestiCarb 作为净化吸附剂。与索氏提取（SE）和超声辅助提取（UAE）相比，QuEChERS 方法易于操作，并且提取得到的提取物更干净。采用 Phenomenex HyperClone BDS C 柱进行 HPLC 分析。甲醇-乙腈-水被选为梯度洗脱的流动相。方法验证表明，所有分析物在考察范围内均表现出良好的线性（r>0.999），回收率在 95.0%至 105.0%之间，令人满意。所建立的 QuEChERS-HPLC 方法简单、高效，适用于测定垂序商陆中的美登素类似物。
