Totakul Pajaree, Matra Maharach, Sommai Sukruthai, Wanapat Metha
Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Anim Biosci. 2021 Oct;34(10):1607-1615. doi: 10.5713/ab.20.0833. Epub 2021 Feb 15.
Chaya (Cnidoscolus aconitifolius) leaf has been found to be an important source of protein, vitamins, minerals, as well as phytonutrients. The present study aimed to evaluate the effect of Chaya leaf pellet (CHYP) with various level of crude protein (CP) in the concentrate on rumen fermentation characteristics and nutrient degradability in in vitro gas production technique.
In an in vitro rumen fermentation study the dietary treatments were arranged according to a 3×5 factorial arrangement in a completely randomized design, consisting of Factor A: three levels of CP of concentrate mixtures (14%, 16%, and 18% CP, respectively) and Factor B: five levels of CHYP supplementation (at 0%, 2%, 4%, 6%, and 8% of dry matter substrates).
The gas production kinetics, fraction (a) and fraction (b) were lower (p<0.05) with an increasing CHYP addition. Additionally, the fraction (a+b) was found to yield a significant interaction (p<0.05) while the fraction (c) was not impacted by CHYP addition. However, in vitro DM degradability was enhanced and interactive (p<0.05), using 16% CP of concentrate with 6% and 8% CHYP, when compared with 18% CP in the non-addition. Additionally, the treatment with higher CP of the concentrate was higher in NH3-N concentration (p<0.001) and by CHYP supplementation group (p<0.05). Nevertheless, protozoal counts in the rumen were remarkably decreased (p<0.05) with increasing level of CHYP supplementation. Furthermore, rumen C2 concentration was lower (p<0.05) in the treatments with CHYP supplementation, while C3 was significantly increased and interactive (p<0.05) between levels of CP and CHYP supplementation especially at 8% CHYP supplementation.
Based on this study, the results revealed CHYP as a promising feed supplement to enhance rumen fermentation and to mitigate methane production. However, in vivo feeding experiments should be subsequently conducted to elucidate the effect of CHYP supplementation on rumen fermentation, as well as ruminant production efficiency.
已发现木薯叶是蛋白质、维生素、矿物质以及植物营养素的重要来源。本研究旨在评估在体外产气技术中，精料中不同粗蛋白（CP）水平的木薯叶颗粒（CHYP）对瘤胃发酵特性和养分降解率的影响。
在一项体外瘤胃发酵研究中，日粮处理按照3×5析因设计进行完全随机排列，包括因素A：精料混合物的三个CP水平（分别为14%、16%和18% CP）和因素B：五个CHYP添加水平（占干物质底物的0%、2%、4%、6%和8%）。
随着CHYP添加量增加，产气动力学、组分（a）和组分（b）降低（p<0.05）。此外，发现组分（a+b）产生显著交互作用（p<0.05），而组分（c）不受CHYP添加影响。然而，与不添加CHYP的18% CP相比，使用含16% CP精料并添加6%和8% CHYP时，体外干物质降解率提高且具有交互作用（p<0.05）。此外，精料中CP水平较高的处理组氨氮浓度更高（p<0.001），CHYP添加组也更高（p<0.05）。然而，随着CHYP添加水平的增加，瘤胃中原虫数量显著减少（p<0.05）。此外，添加CHYP的处理组瘤胃中C2浓度较低（p<0.05），而C3显著增加，且在CP水平和CHYP添加水平之间具有交互作用（p<0.05），尤其是在添加8% CHYP时。
基于本研究，结果表明CHYP是一种有前景的饲料添加剂，可增强瘤胃发酵并减少甲烷产生。然而，随后应进行体内饲养实验，以阐明添加CHYP对瘤胃发酵以及反刍动物生产效率的影响。
