Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235, s/n, Setor Universitário, Goiânia, Goiás CEP: 74605-050, Brazil; Programa de Pós-graduação em Ciências Animal, Universidade Federal de Goiás, Avenida Esperança, s/n, Campus Samambaia, Goiânia, Goiás CEP: 74.690-900, Brazil.
Grupo de Química Supramolecular e Biomimética (GQSB), Departamento de Química, Universidade Federal de Viçosa (UFV), Campus Universitário, Avenida P.H. Rolfs, s/n, Viçosa, Minas Gerais CEP: 36570-900, Brazil.
Vet Parasitol Reg Stud Reports. 2021 Jan;23:100526. doi: 10.1016/j.vprsr.2020.100526. Epub 2020 Dec 25.
This study aimed to evaluate the combination effect of Heterorhabditis bacteriophora HP88 and H. indica LPP1, with the acaricides deltamethrin, amitraz and chlorfenvinphos, and the essential oil (EO) of Lippia triplinervis, against engorged females of Rhipicephalus microplus. In order to verify the effect of acaricides and EO, the adult immersion test was used, and in the groups treated only with entomopathogenic nematodes (EPNs), 150 infective juveniles were used per female. In the treatments with nematodes in combination with the acaricides or EO, the females were immersed in the solutions (acaricide or EO) and then transferred to Petri dishes for application of the nematodes. The treatment with acaricides resulted in a control percentage lower than 70%, except in the group treated with chlorfenvinphos in the second experiment (84.3%). The control percentage was 73% for L. triplinervis EO, and greater than 90% in all the groups treated with nematodes. For treatments with EPNs combined with the acaricides or EO, the efficacy was greater than 95% (except for deltamethrin + HP88), and reached 100% in the treatment with LPP1 + amitraz. It can be concluded that the EPNs at the concentrations tested were compatible with the acaricides deltamethrin, amitraz and chlorfenvinphos, and with the EO of L. triplinervis. These combinations enhance the effect of these control agents.
本研究旨在评估异小杆线虫 HP88 和印度异小杆线虫 LPP1 与杀螨剂溴氰菊酯、双甲脒和氯氟磷，以及薰衣草精油对饱血雌蜱的联合作用。为了验证杀螨剂和精油的效果，采用了成螨浸浴试验，并且在仅用昆虫病原线虫（EPN）处理的组中，每头雌蜱使用 150 条侵染性幼虫。在与杀螨剂或精油联合处理的线虫中，将雌蜱浸入溶液（杀螨剂或精油）中，然后转移到培养皿中应用线虫。除第二次实验中用氯氟磷处理的组（84.3%）外，杀螨剂处理的控制率均低于 70%。薰衣草精油的控制率为 73%，而所有用线虫处理的组的控制率均高于 90%。对于与杀螨剂或精油联合使用的 EPN 处理，疗效均大于 95%（除了溴氰菊酯+HP88 外），并且在用 LPP1+双甲脒处理时达到 100%。可以得出结论，在所测试的浓度下，EPN 与杀螨剂溴氰菊酯、双甲脒和氯氟磷以及薰衣草精油兼容。这些组合增强了这些控制剂的效果。
