Biodiversity of meatborne spp. in Himachal Pradesh and their interaction with indigenous probiotics.

This study determined the anti-listerial activity of indigenous probiotics from traditional fermented foods of Western Himalaya against meat borne isolates from Himachal Pradesh. One hundred samples of meat and meat products like chicken (n = 25), chevon (goat meat, n = 20), fish (n = 20) and pork (n = 30) were collected and were analyzed for the presence of spp. by recommended culture and biochemical methods. isolates were confirmed by PCR targeting the virulence gene (haemolysin A) and by16S rRNA sequencing. Anti-listerial activity of probiotic bacteria isolated from indigenous fermented foods of Himachal Pradesh was determined by well diffusion method using (ATCC 53103) as the reference strain. Five percent of tested samples were found positive for with incidence of 8.0% in chicken (2/25), 10.0% in fish (2/20) and 4.0% in chevon meat (1/25). None of the tested pork samples were found contaminated with . Among 11 indigenous probiotics used in this study, highest antagonistic activity was exhibited by (ADF 10) and (ADF1) which was equivalent to the reference strain.